| 包埋厌氧氨氧化菌的环境因子影响特性及群落结构分析 |
| 摘要点击 1648 全文点击 646 投稿时间：2019-08-19 修订日期：2019-09-16 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 包埋 聚乙烯醇-聚丙烯(PVA-PP) 脱氮特性 微生物群落结构 |
| 英文关键词 anaerobic ammonia oxidizing(ANAMMOX) immobilization polyvinyl alcohol-polypropylene (PVA-PP) nitrogen removal characteristics microbial community structure |
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| 中文摘要 |
| 针对厌氧氨氧化菌悬浮培养启动慢、易流失、活性易受环境因子抑制等问题，利用聚乙烯醇-聚丙烯（PVA-PP）制备厌氧氨氧化菌包埋填料，在实现厌氧氨氧化菌活性提高及反应体系稳定运行的基础上，采用批次实验明确了COD干扰、pH值变化及摇床转速对包埋填料脱氮特性的影响.并通过高通量测序技术分析了填料内菌群结构和多样性的变化.结果表明，厌氧氨氧化菌活性可在第30 d恢复至100%，阶段培养99 d，总氮容积负荷（NLR）为0.69 kg·（m3·d）-1时，总氮去除率为87.7%，140 d长期运行，总氮去除速率（NRR）可达1.83 kg·（m3·d）-1，是包埋前悬浮污泥的9.4倍.种群多样性在包埋载体内得到保持，厌氧氨氧化功能菌Candidatus Kuenenia（AF375995.1）有效富集，占比由11.06%上升至32.55%.PVA-PP包埋载体可实现厌氧氨氧化-反硝化耦合脱氮，有机碳源干扰及pH值的变化对厌氧氨氧化菌影响抑制明显减弱，并且摇床转速的适当提高会促进包埋体系厌氧氨氧化反应的进行. |
| 英文摘要 |
| To solve the problems of a slow start, easy loss, and easily inhibited activity of the Anammox bacteria suspension culture, polyvinyl alcohol-polypropylene (PVA-PP) was used to prepare the Anammox immobilized filler. To improve the activity of Anammox bacteria and ensure stable operation of the reaction system, the effects of COD interference, change in pH value, and rotating speed on the nitrogen removal characteristics of the immobilized filler were determined in batch tests. Changes in the structure and diversity of the bacteria in the filler were analyzed by a high-throughput sequencing technique. The results showed that the activity of Anammox bacteria could recover to 100% on the 30th day, and the total nitrogen removal rate was 87.7% when the total nitrogen volume load (NLR) was 0.69 kg·(m3·d)-1 at the stage of 99 days. After 140 days of long-term operation, the total nitrogen removal rate (NRR) reached 1.83 kg·(m3·d)-1, which was 9.4 times the suspended sludge before immobilization. The diversity of the population was maintained in the inclusion carrier, and the effective enrichment of Candidatus Kuenenia (AF375995.1), which performs anaerobic ammonia-oxidization, increased from 11.06% to 32.55%. The influence of COD interference and changes in the pH value of Anammox bacteria was significantly weakened, and the PVA-PP entrapped carrier could achieve the coupling removal of nitrogen by Anammox and denitrification. Appropriate external hydraulic disturbance would promote the Anammox reaction in immobilized systems. |
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