| 静压快速启动亚硝酸盐依赖型甲烷厌氧氧化反应 |
| 摘要点击 1344 全文点击 480 投稿时间：2018-05-18 修订日期：2018-06-11 |
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| 中文关键词 亚硝酸盐依赖型甲烷厌氧氧化(N-DAMO) 快速启动 静压 M.oxyfera 序批式活性污泥反应器(SBR) |
| 英文关键词 nitrite-dependent anaerobic methane oxidation (N-DAMO) rapid start-up static pressure M. oxyfera sequencing batch reactor (SBR) |
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| 中文摘要 |
| 在序批式反应器（SBR）中接种污水厂厌氧池污泥和深层水稻土的1：1混合污泥，在阶段性提升基质NO2--N浓度的条件下，考察静压条件对亚硝酸盐依赖型甲烷厌氧氧化（nitrite-dependent anaerobic methane oxidation，N-DAMO）反应快速启动过程的影响，并对N-DAMO反应的主导微生物的丰度变化进行分析.结果表明运行120 d后，常压（R1）和0.3 MPa静压（R2）条件下，都观测到了明显的N-DAMO现象，并且R2的NO2--N去除速率达到了36.90 mg·（L·d）-1，较R1提高了24%；其4 h内平均脱氮速率（以NO2--N计）达到0.10 mmol·（L·h）-1，比R1提高了186%.R2中富集得到的污泥粒径约为R1的2倍，比表面积更大，优化了污泥中甲烷的传质情况，有助于N-DAMO反应的进行，且R2污泥的N-DAMO比活性（以N/VSS计）达到了0.29 mg·（g·h）-1，是R1的2倍.此外，静压有助于N-DAMO功能微生物Candidatus Methylomirabilish oxyfera（M.oxyfera）的生长，实验结束时R2中M.oxyfera细菌16S rRNA基因的丰度比接种初期提高了22倍，是同阶段R1中的10倍.可见，提高静压能够有效促进N-DAMO反应的启动. |
| 英文摘要 |
| The present study explores the effect of static pressure on the rapid start-up of a nitrite-dependent anaerobic methane oxidation (N-DAMO) process in lab-scale sequencing batch reactors (SBR). A mixture of anaerobic sludge and deep paddy soil with a volume ratio of 1:1 was used as inoculum and the influent of the nitrite (NO2--N) concentration was gradually increased to avoid a toxicity shock. The variation of the NO2--N removal performance and corresponding microbial characteristics were analyzed to evaluate the development of the N-DAMO process. After 120 days of operation, significant N-DAMO phenomena were observed in both the control SBR (R1) with normal pressure and pressurized SBR (R2) with a static pressure of 0.3 MPa. The NO2--N removal rate (measured by NO2--N) of R2 (36.90 mg·(L·d)-1) was 24% higher than that of R1, while the average NO2--N removal rate in the first 4 h of the batch cycle in R2 (0.10 mmol·(L·h)-1) was 186% higher than that of R1. The mean sludge size of R2 was~2-fold larger than that of R1. Sludge in R2 also has a bigger specific surface area, which improves the mass transfer rate of methane and the N-DAMO performance. The specific activity of N-DAMO (measured by N/VSS) reached 0.29 mg·(g·h)-1 in the study period, which is approximately 2 times higher than that of R1. Moreover, the abundance of N-DAMO functional microbes Candidatus Methylomirabilish oxyfera (M. oxyfera) in R2 was 10-fold higher than that of R1. These results indicate that static pressure effectively accelerates the start-up of the N-DAMO process. |
