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1株耐盐异养硝化-好氧反硝化菌Zobellella sp.B307的分离及脱氮特性
摘要点击 243  全文点击 69  投稿时间:2018-02-02  修订日期:2018-04-01
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中文关键词  耐盐  异养硝化  好氧反硝化  脱氮  Zobellella sp.
英文关键词  salt-tolerance  heterotrophic nitrification  aerobic denitrification  nitrogen removal  Zobellella sp.
作者单位E-mail
白洁 中国海洋大学环境科学与工程学院, 青岛 266100
中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100 
baijie@ouc.edu.cn 
陈琳 中国海洋大学环境科学与工程学院, 青岛 266100  
黄潇 哈尔滨工业大学深圳研究生院土木与环境工程学院, 深圳 518055  
胡春辉 青岛农业大学生命科学学院, 青岛 266109  
赵阳国 中国海洋大学环境科学与工程学院, 青岛 266100
中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100 
 
李岿然 中国海洋大学海洋生命学院, 青岛 266003 likr@ouc.edu.cn 
中文摘要
      从胶州湾沉积物中分离出1株异养硝化-好氧反硝化菌株B307,采用16S rRNA基因序列分析对该菌株进行鉴定,采用单因素实验对其进行条件优化和耐盐特性研究,并在最优条件下考察其在单一和混合氮源中的脱氮效果.结果表明,该菌为Zobellella sp.,其最佳碳源为丁二酸钠,最适C/N为5,最适初始pH为9,最适温度为35~40℃.该菌株在混合氮源体系中12 h对NH4+-N和NO3--N的去除率分别为98.35%和99.75%;在盐度为75 g·L-1(以NaCl计)条件下24 h对NH4+-N和NO3--N去除率仍分别保持在97.67%和94.39%.表明该菌株具有高效的异养硝化-好氧反硝化能力和较强的耐盐特性,在高盐废水脱氮等领域具有广泛的应用前景.
英文摘要
      A heterotrophic nitrification and aerobic denitrification strain, B307, was isolated from the sediment of Jiaozhou Bay. The strain was identified by 16S rRNA sequence analysis, and its optimization condition and salt-tolerance characteristics were studied by single factor experiment. The denitrification effect in single or mixed nitrogen sources was investigated under optimum conditions. The results allowed the strain to be identified as Zobellella sp., based on 16S rRNA sequence analysis. The best carbon source was sodium succinate, and the optimum C/N was 5, the optimal initial pH was 9, and the optimal temperature was 35-40℃ respectively. After 12 hours, the NH4+-N and the NO3--N removal efficiencies were 98.35% and 99.75% in a mixed nitrogen source system. The removal efficiencies for NH4+-N and NO3--N were 97.67% and 94.39% within 24 hours when salinity was 75 g·L-1. The strain has highly efficient heterotrophic nitrification-aerobic denitrification ability and strong salt tolerance, which demonstrated that the strain has potential for extensive application for nitrogen removal in high salt wastewater.

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