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碧流河水库细菌群落结构特征及其关键驱动因子
摘要点击 46  全文点击 19  投稿时间:2017-11-02  修订日期:2018-02-24
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中文关键词  碧流河水库  细菌  16S rDNA  环境因子  RDA
英文关键词  Biliuhe Reservoir  bacteria  16S rDNA  environmental factors  RDA
作者单位E-mail
王欢 大连海洋大学水产与生命学院, 辽宁省水生生物学重点实验室, 大连 116023 897694663@qq.com 
赵文 大连海洋大学水产与生命学院, 辽宁省水生生物学重点实验室, 大连 116023 zhaowen_1963@163.com 
谢在刚 大连市碧流河水库管理局, 大连 116221  
魏杰 大连海洋大学水产与生命学院, 辽宁省水生生物学重点实验室, 大连 116023  
姜洋 大连海洋大学水产与生命学院, 辽宁省水生生物学重点实验室, 大连 116023  
中文摘要
      为了解碧流河水库细菌群落结构及其多样性变化,探讨环境因子对其的影响,于2016~2017年应用16S rDNA高通量测序技术,分析比较了碧流河水库一年周期内4个季节的细菌群落结构及其多样性差异,使用冗余分析(redundancy analysis,RDA)探讨水体环境因子与细菌群落结构及其多样性的关系.结果表明,全年碧流河水库细菌群落多样性指数(Shannon-Wiener指数)平均达6.9,冬季 < 夏季 < 春季 < 秋季.序列比对结果显示,除了分类地位不明确的菌群和稀有菌群外,共发现了细菌44门115纲184目368科865属,优势菌门为变形菌门Proteobacteria 34.16%,蓝菌门Cyanobacteria 24.35%,放线菌门Actinobacteria 13.35%,拟杆菌门Bacteroidetes 12.42%.优势菌门变形菌门中优势菌纲为β-变形菌纲β-Proteobacteria 18.34%,优势菌属为unidentified_Chloroplast 17.73%;RDA结果表明不同菌群受环境因子影响不同,悬浮物(TSS)、透明度(SD)、酸碱度(pH)、活性磷(ADP)、溶氧(DO)、总磷(TP)、亚硝酸氮(NO2--N)为影响群落分布主要的环境因子,其中夏季菌群与NO2--N、ADP、TP和TSS显著正相关.
英文摘要
      In order to understand the bacterial community structure and its diversity in the Biliuhe Reservoir, the impact of environmental factors must be explored. Using 16S rDNA high-throughput sequencing technology, the bacterial community structure and diversity differences in four seasons were analyzed and compared from 2016 to 2017. Redundancy analysis (RDA) was used to investigate the relationship between the environmental factors in water and bacterial diversity. The analysis of bacterial diversity index (Shannon-Wiener index) showed that the bacterial community diversity index reached 6.9 in the whole year, with the following pattern:Winter < summer < spring < autumn. Sequence alignment results show that although the flora status was not clear and rare flora were seen, a total of 44 bacterial phyla, 115 classes, 184 orders, 368 families, and 865 genera were found. The dominant bacteria phyla were:Proteobacteria (34.16%), Cyanobacteria (24.35%), Actinobacteria (13.35%), Bacteroidetes (12.42%). The dominant class of bacteria in the dominant phylum (Proteobacteria) was β-Proteobacteria (18.34%), and the dominant strain was unidentified_Chloroplast (17.73%). The results of RDA showed that different bacterial communities were affected by environmental factors. Total suspended solids (TSS), transparency (SD), pH, active phosphorus (ADP), dissolved oxygen (DO), total phosphorus (TP), and nitrite nitrogen (NO2--N) were the main environmental factors influencing community distribution. The bacterial community during the summer was positively correlated with NO2--N, ADP, TP, and TSS.

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