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澜沧江流域浮游细菌群落结构特征及驱动因子分析
摘要点击 252  全文点击 82  投稿时间:2017-11-01  修订日期:2018-02-08
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中文关键词  澜沧江  高通量测序  细菌多样性  群落结构  环境因子
英文关键词  Lancang River  high-throughput sequencing  bacterial diversity  community structure  environmental factors
作者单位E-mail
程豹 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068 554042749@qq.com 
望雪 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068  
徐雅倩 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068  
杨正健 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068 656637841@qq.com 
刘德富 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068  
马骏 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068  
中文摘要
      为探究澜沧江流域的浮游细菌群落结构特征及驱动因子,应用16S rRNA高通量测序技术,分析了2017年2月澜沧江流域浮游细菌群落结构特征,并采用Pearson相关性分析(Pearson correlation analysis)和冗余分析(RDA)识别了澜沧江自然河道段和水库段浮游细菌群落结构变化的关键环境因子.结果表明,自然河道段ACE指数和Shannon指数均高于水库段,造成自然河道段和水库段浮游细菌多样性变化的主要环境因子为水温(WT)、溶解氧(DO)、浊度(Tur)、高锰酸盐指数(permanganate index)、pH和总氮(TN).对16S rRNA V3和V4测序,得到用于物种分类的OTU数共26772,涵盖了浮游细菌群落共45门,965属.菌群分类发现,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)为优势门,其中变形菌门(Proteobacteria)含量相对丰富,占细菌群落的36%~94%.澜沧江流域变形菌门(Proteobacteria)主要包括α-变形菌纲(α-Proteobacteria)、β-变形菌纲(β-Proteobacteria)和γ-变形菌纲(γ-Proteobacteria),分别占变形菌门(Proteobacteria)的比例为0.39%~21.56%、0.39%~55.80%和31.09%~99.18%.澜沧江水体浮游细菌群落空间差异明显,影响浮游细菌群落结构变化的环境因子主要为WT、高锰酸盐指数、Tur、DO和TN.自然河道段和水库段影响浮游细菌群落结构的环境因子不同,DO和Tur是影响自然河道段浮游细菌群落结构的关键环境因子,而水库段浮游细菌群落结构主要受WT、高锰酸盐指数和TN的影响.
英文摘要
      In order to explore the bacterioplankton diversity community structure in the Lancang River basin, as well as to study the relationship between the bacterial diversity and environmental factors, MiSeq high-throughput sequencing was used to analyze and compare the bacterial diversity and community composition of samples in February 2017. Furthermore, Pearson correlation analysis and redundancy analysis (RDA) were used to identify the key environmental factors for the change of bacterial community structure in the natural river and reservoirs of the Lancang river. The results show that the ACE index and Shannon index of the natural river are higher than those of the reservoir, and the main environmental factors causing the diversity of planktonic bacteria in natural river and reservoir segments are water temperature (WT), dissolved oxygen (DO), turbidity (Tur), permanganate index, pH, and total nitrogen (TN). Overall, 26772 optimized reads were obtained from samples based on the high-throughput sequencing of the V3 and V4 region of the 16S rRNA gene. The bacterial species detected in these samples cover 45 phyla and 965 genera. Taxonomic assignment analysis indicated that Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria are the abundant phyla. Among them, the Proteobacteria content is relatively rich, accounting for 36%-94% of the bacterial community. The predominant groups are α-Proteobacteria, β-Proteobacteria, and γ-Proteobacteria, at the genus level, with Proteobacteria ratios of 0.39%-21.56%, 0.39%-55.80% and 31.09%-99.18%, respectively. The environmental factors affecting the community structure of planktonic bacteria are different in natural river and reservoir. DO and Tur are the key environmental factors affecting the community structure of planktonic bacteria in the natural river, while the community structure of planktonic bacteria in reservoirs is mainly affected by WT, permanganate index, and TN.

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