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硝化细菌的培养及包埋固定化中试
摘要点击 233  全文点击 61  投稿时间:2017-10-16  修订日期:2017-12-04
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中文关键词  硝化细菌  富集培养  细菌包埋  扩大培养  高通量测序
英文关键词  nitrifying bacteria  enrichment culture  embedding immobilization  intermediate culture  high-throughput sequencing
作者单位E-mail
杨宏 北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124 yhong@bjut.edu.cn 
胡银龙 北京工业大学水质科学与水环境恢复工程北京市重点实验室, 北京 100124  
中文摘要
      为实现硝化细菌规模化富集及包埋固定化技术的工业化应用.以污水厂回流污泥为菌源,利用工业级生物发酵罐,连续投加并逐渐提高底物浓度,控制FA和FNA实现硝化细菌的快速增长,实现了氨氧化速率118 mg·(L·h)-1的高表达.高通量种群分析结果表明,回流污泥生物多样性较大,具有硝化作用的Nitrosomonas比例仅为0.53%;富集培养后污泥多样性明显降低,Nitrosomonas比例上升至10.27%,相较于驯化前,比例提高了20倍.以此为菌源,用PVA(聚乙烯醇)进行包埋固定化,包埋填料填充率为30%,通过连续流的方式进行包埋填料的活性恢复,仅用27 d填料的硝化速率达到62 mg·(L·h)-1,证明包埋填料活性恢复.
英文摘要
      In order to realize the industrialization of nitrifying bacteria enrichment cultivation and large-scale application of entrapment immobilization, activated sludge from a sewage treatment plant was used as the seed sludge. The concentration of substrate was increased progressively in an industrialized tank. Nitrifying bacteria were grown rapidly by controlling the concentration of FA and FNA, and the ammonia oxidation rate reached 118 mg·(L·h)-1 eventually. The results of high-throughput sequencing showed that Nitrosomonas, nitrifying bacteria, was only 0.53% of the bacteria. After exposing it to an enrichment culture, the content of Nitrosomonas rose to 10.27%, 20 times larger than that in the raw sludge. Diversity was significantly smaller at the same time. After embedding immobilization with PVA (polyvinyl alcohol), the embedded fillers nitrification load was 30%. A continuous-flow approach was utilized in the recovery phase, and the nitrification loading rate reached a high level, 62 mg·(L·h)-1, and efficient recovery of the biologically active fillers was achieved.

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