| 环境因子对全自养脱氮颗粒污泥功能菌协同效应的影响 |
| 摘要点击 1895 全文点击 620 投稿时间：2017-08-31 修订日期：2017-09-11 |
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| 中文关键词 颗粒污泥 亚硝化 厌氧氨氧化 环境因子 协同效应 |
| 英文关键词 granular sludge partial nitritation ANAMMOX environmental factor synergy effect |
| 作者 | 单位 | E-mail | | 陈希 | 苏州科技大学环境科学与工程学院, 苏州 215009 | 1074465572@qq.com | | 钱飞跃 | 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | | | 王建芳 | 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | wjf302@163.com | | 高军军 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 沈耀良 | 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | | | 贾珣 | 苏州科技大学环境科学与工程学院, 苏州 215009 | |
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| 中文摘要 |
| 以全自养脱氮颗粒污泥为研究对象，在采用MiSeq高通量测序技术探明其微生物菌群结构的基础上，通过单因子批次实验，系统考察了溶解氧（DO）浓度、反应温度（t）、初始氨氮（NH4+-N）浓度和溶液pH对好氧与厌氧氨氧化菌（AOB和AMX）之间协同作用的影响，以期为新工艺的运行调控提供理论参考.结果表明，以Nitrosomonas属（相对丰度32.9%）和Candidatus Kuenenia属（相对丰度9.8%）为代表的AOB和AMX在颗粒污泥中占据优势地位.当初始NH4+-N浓度为100 mg·L-1时，颗粒污泥的总氮比降解速率[q（TN）]在DO=2 mg·L-1时达到最大值（17.7±1.0）mg·（g·h）-1.过低或过高的DO浓度将分别导致亚硝化和厌氧氨氧化成为脱氮的限速步骤.依据反应自由能可知，AMX活性较AOB更易受到低温条件的抑制.当t<30℃时，系统中出现亚硝态氮累积现象，q（TN）值显著降低.在相同的供氧条件下，初始NH4+-N浓度低于100 mg·L-1不能充分发挥污泥中AMX的脱氮能力.但当初始NH4+-N浓度超过150 mg·L-1时，供氧不足和高游离氨又会导致q（TN）值的持续下降.此外，颗粒污泥中两类氨氧化菌在pH 7.0~8.5范围内都表现出了良好的协同作用. |
| 英文摘要 |
| To obtain experimental evidences for optimizing a completely autotrophic nitrogen removal process based on granules, the effects of dissolved oxygen (DO) concentration, temperature (t), initial ammonium (NH4+-N) concentration, and solution pH conditions on the synergy between the aerobic and anaerobic ammonium-oxidizing bacteria (AOB and AMX) were investigated using a single factor batch experiment, while the analysis of the microbial community structure within them was conducted using MiSeq high-throughput pyrosequencing. Results revealed that AOB (genus Nitrosomonas) and AMX (genus Candidatus Kuenenia) dominated in the granules, representing relative abundances of 32.9% and 9.8%, respectively. For the granules, the highest specific nitrogen removal rate of q(TN)=(17.7±1.0) mg·(g·h)-1 was obtained at a DO concentration of 2 mg·L-1, while the initial NH4+-N concentration was set at 100 mg·L-1. And a lower DO level resulted in partial nitritation became the rate-limiting step of process, otherwise, it would be the ANAMMOX reaction instead. According to the free energy of the reactions, the activity of AMX was more sensitive to low temperature than that of AOB. When the reaction temperature was lower than 30℃, nitrite accumulation could be observed in bulk liquid, with the significant decrease of q(TN) for the granules. Under the same oxygen supply conditions, an initial NH4+-N concentration lower than 100 mg·L-1 could inhibit the activity of AMX partly. However, with an initial NH4+-N concentration over 150 mg·L-1, either oxygen-limiting or high free ammonia concentration could lead to the dramatic decrease of q(TN). In addition, the effective synergy of the two types of ammonium oxidizers in granules was always achieved at solution pH in the range of 7.0-8.5. |
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