| 喀斯特灌丛土壤丛枝菌根真菌群落结构及丰度的影响因子 |
| 摘要点击 2162 全文点击 677 投稿时间：2017-04-14 修订日期：2017-05-27 |
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| 中文关键词 喀斯特 灌丛生态系统 坡位 丛枝菌根真菌 群落结构 |
| 英文关键词 karst shrub ecosystem slope position arbuscular mycorrhizal fungi community structure |
| 作者 | 单位 | E-mail | | 梁月明 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125
中国地质科学院岩溶地质研究所, 国土资源部、广西壮族自治区岩溶动力学重点实验室, 桂林 541000 | yueming0919@163.com | | 苏以荣 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | yrsu@isa.ac.cn | | 何寻阳 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 陈香碧 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 胡亚军 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | |
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| 中文摘要 |
| 运用末端限制性片段长度多态性（terminal restriction fragment length polymorphism，T-RFLP）和荧光定量PCR（real-time PCR）法，检测喀斯特灌丛生态系统中不同坡位条件下土壤丛枝菌根（arbuscular mycorrhizal，AM）真菌群落结构与丰度的变化，揭示不同坡位条件下影响灌丛生态系统土壤AM真菌群落结构与丰度的关键因子.研究结果表明不同坡位条件下，土壤有机碳、全氮和速效磷含量差异显著，变化趋势为上坡位≈中坡位>下坡位；土壤AM真菌丰度变化趋势为上坡位≈中坡位<下坡位.相关性分析表明，土壤速效磷含量与AM真菌丰度存在显著负相关（P<0.05）. RDA分析表明不同坡位条件下，土壤AM真菌与植物群落结构存在显著差异，植物均匀度指数显著影响土壤AM群落结构组成，而土壤有机碳、全氮显著影响植物群落结构组成.可见，不同坡位条件下，土壤养分与植物群落结构共同作用影响土壤AM真菌群落结构，说明利用AM真菌用于喀斯特地区植被恢复时，微形态（坡位）对AM真菌的影响应该纳入考虑范畴. |
| 英文摘要 |
| Slope position is a key factor used in the restoration of vegetation in degraded karst ecosystems, and arbuscular mycorrhizal fungi (AMF) play an important role in improving this plant growth. However, little information is available regarding the effects of slope position on arbuscular mycorrhizal fungi. To test whether these fungal communities are impacted by slope position, the abundance, and composition of soil, AMF communities along the slope position were analyzed through terminal restriction fragment length polymorphism (T-RFLP) and real-time fluorescence-based quantitative polymerase chain reaction (real-time PCR). The diversity, richness, and evenness of plant species were evaluated through field surveys and soil properties were also measured. The results show that content of carbon, nitrogen, and phosphorus in the soil are different along the slope, and the trends identified were that the upper slope position ≈ middle slope position > lower slope position. The trend for AMF abundance was identified as upper slope position ≈ middle slope position < lower slope position. The available phosphorus content in the soil correlated significantly with the AMF abundance. A redundancy analysis showed that the structure of soil, AMF, and plant community compositions differed along the slope. The plant evenness index was shown to significantly contribute to the distribution of the AMF community structure, while the total nitrogen and total organic carbon content of the soil had a significant effect on the plant community structure. These results indicate that the interaction effects of soil nutrients and plant community structures on the soil AMF community structures suggest micro-morphology should be taken into account when AMF is used to restore vegetation in karst regions. |
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