| 采用颜色空间表征ANAMMOX启动与冲击过程 |
| 摘要点击 1735 全文点击 598 投稿时间：2017-01-16 修订日期：2017-03-15 |
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| 中文关键词 厌氧氨氧化 颜色空间 高通量测序 图像分析 负荷冲击 |
| 英文关键词 ANAMMOX color space high-throughput sequencing image analysis load impact |
| 作者 | 单位 | E-mail | | 王利君 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055 | wanglijun919@163.com | | 李志华 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055 | lizhihua@xauat.edu.cn | | 韩冬 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 张天宇 | 蒙大拿州立大学数学科学系, 美国蒙大拿州, 博兹曼 59717 | | | 杨成建 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055 | |
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| 中文摘要 |
| 厌氧氨氧化（ANAMMOX）是一种高效、节能的脱氮技术，但是其启动过程和稳定状态的表征目前仍缺乏一个简单易行的方法. 针对这一问题，在水质分析的基础上，采用颜色空间重点监测ANAMMOX启动过程中污泥的颜色变化，并借助高通量测序技术对微生物菌群的种类和数量等进行分析. 结果表明：①根据水质特征，整个启动过程可分为活性迟滞期、活性增强期、负荷提高期、稳定运行期. 与此同时，HSV、CIELAB颜色空间的指标，整体上呈现先降低后升高的趋势，然后保持稳定. 污泥颜色的变化与水质的变化以及分子生物学特征中优势菌群的变迁一致，因此，三者之间具有相关性，进而表明，采用颜色空间可对ANAMMOX启动过程进行准确表征；②当遇到高负荷引起的冲击时，H、S、a*、b*、Cab*等指标与总无机氮（TIN）容积去除速率均呈现降低的趋势，而Hab出现了突升，但均能准确反映出系统的冲击特性. 提出了基于颜色空间表征ANAMMOX启动各阶段的方法并建立了一套冲击过程指标体系，为颜色空间在ANAMMOX系统中的应用提供了理论基础. |
| 英文摘要 |
| Anaerobic ammonia oxidation (ANAMMOX) is an efficient and energy-saving denitrification technology, but it still lacks a simple and easy method to characterize its start-up process and stable state. Based on the analysis of water quality, color space was used to monitor the color change of sludge during the start-up of ANAMMOX, and the species and quantity of microbial flora were analyzed by high-throughput sequencing technology. The results were as follows. ① According to water quality characteristics, the whole start-up process can be divided into activity lag phase, activity enhancement period, load increase period, and stable operation period. At the same time, HSV and CIELAB color space indicators decreased first, then increased, and finally remained stable. The change of sludge color was consistent with the change of water quality and the change of dominant bacteria based on the molecular biology testing, which implies correlations among these three. Therefore, color space can be used to characterize the start-up process of ANAMMOX. ② When subjected to shocks caused by high load, H, S, a*, b*, Cab*, and TIN volume removal rate all decreased, while Hab suddenly increased. The impact characteristic of the system was accurately expressed by all of the color indicators. This paper proposes a color space-based method for characterizing all phases of the start-up of ANAMMOX and a shock process index system that provides a theoretical basis for applying color space in ANAMMOX systems. |
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