| 不同湿地模型中根系微生物的多样性 |
| 摘要点击 1487 全文点击 615 投稿时间：2017-01-04 修订日期：2017-03-04 |
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| 中文关键词 湿地模型 根际 高通量测序技术 微生物群落 水质分析 β-变形菌纲 |
| 英文关键词 wetland microcosm rhizosphere high-throughput sequencing technology microbial community water quality analysis β-Proteobacteria |
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| 中文摘要 |
| 为了研究不同植物和滤料组合的小型湿地模型在处理养殖池塘水体过程中根系微生物群落的特征，并分析植物对根系微生物富集的影响，本研究对两种植物（茭白和慈菇）和两种滤料（石榴石和磁铁矿）进行组合构建了6种不同的湿地模型，并利用高通量测序技术分析湿地植物根系微生物的结构特点和多样性. 结果表明：不同湿地模型根系微生物的种类和数量各不相同；所有收集的微生物总体上属于52个门，118个纲，455科，905个属和1426个种；根据Shannon指数，茭白（平均为5.77）比慈菇（平均为5.29）在微生物富集方面具有更强的能力；但是，慈菇和茭白根系微生物中变形菌门的比例分别平均为61.97%和51.78%，说明慈菇能够更好地富集变形菌. β-变形菌纲为实验期间不同植物根际富集的主要细菌类群. 本研究结果为人工湿地植物优化选择提供了理论基础和湿地组合最佳构建提供了基础资料. |
| 英文摘要 |
| To investigate the rhizosphere microbial community structure characteristics in varying combinations of plants and filter materials in the process of aquaculture pond water treatment, six types of wetland microcosms were established using two kinds of plants (Zizania latifolia Turcz. and Sagittaria graminea) and two types of filter materials (garnet and magnetite). High-throughput sequencing technology was used to analyze the microbial structural features and composition diversity of the wetland rhizosphere. The results were as follows. The microorganisms recovered belonged to 52 phyla, 118 classes, 455 families, 905 genera, and 1426 species. Based on the Shannon index, Zizania latifolia Turcz. (average value: 5.77) had a higher capacity than Sagittaria graminea(average value: 5.29) in terms of microbial enrichment. However, the rate of Proteobacteria in the rhizosphere microorganism communities of the Sagittaria graminea and Zizania latifolia Turcz. microcosms were 61.97% and 51.78%, respectively, further showing that the roots of Sagittaria graminea in the wetland microcosms better enriched the Proteobacteria during the experimental period. The major bacterial groups of different plant roots were enriched with β-Proteobacteria in the experiment. The results of this study provide a theoretical basis for species optimization in artificial wetland systems and best combination of wetland construction that will be useful for future investigations. |
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