| 活性炭在中高温条件下对玉米秸秆厌氧发酵的影响 |
| 摘要点击 2161 全文点击 1078 投稿时间：2016-07-28 修订日期：2016-12-30 |
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| 中文关键词 玉米秸秆 活性炭 厌氧发酵 优势菌种 载体 |
| 英文关键词 corn straw activated carbon anaerobic fermentation dominant bacteria carrier |
| 作者 | 单位 | E-mail | | 甘荣 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | ganrong@njtech.edu.cn | | 葛明民 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | | | 刘勇迪 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | | | 贾红华 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | | | 闫志英 | 中国科学院成都生物研究所, 成都 610041 | | | 雍晓雨 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | | | 吴夏芫 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | | | 周俊 | 南京工业大学生物与制药工程学院, 南京 211816
南京工业大学生物能源研究所, 南京 211816 | zhoujun@njtech.edu.cn |
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| 中文摘要 |
| 为提高玉米秸秆厌氧发酵的产气效果，本文研究活性炭在中温（38℃）和高温（50℃）条件下对玉米秸秆厌氧发酵产甲烷的效果及微生物学机制.结果表明，添加活性炭能显著促进秸秆厌氧发酵产甲烷，中、高温试验组（添加活性炭）的累积产甲烷量分别比对照提高了63%和96%；DGGE的结果显示，高温试验组（添加活性炭）和对照组（未添加活性炭）的发酵液中的优势细菌菌群分别是Clostridiale bacterium和Bacillus，中温对照组发酵液和中温试验组发酵液未发现明显优势菌种.添加活性炭分别有利于氢营养型的甲烷鬃毛菌（Methanosaeta concilii strain）和乙酸营养型的醋酸甲烷八叠球菌（Methanosarcina acetivorans strain），在中温、高温试验组的发酵液中形成优势古菌菌群.中温试验组活性炭载体上的优势古菌菌群为甲烷鬃毛菌（Methanosaeta concilii strain），而高温试验组活性炭载体上的优势古菌菌群主要为甲烷八叠球菌嗜高温菌属（Methanosarcina thermophila strain）. |
| 英文摘要 |
| In order to improve the methane production and concentration, effect of activated carbon addition on the anaerobic fermentation of corn straw under the conditions of mesophilic temperature (38℃) and thermophilic temperature(50℃) was investigated in this study. The results showed that the addition of activated carbon could significantly promote methane production. Compared with the control group in mesophilic and thermophilic conditions, cumulative methane production could be increased by 63% and 96% in test groups. By DGGE analysis, the bacterium enriched by addition of activated carbon was mainly Clostridiales bacterium, compared to Bacillus (without adding activated carbon) in the thermophilic system, while the differences in fermentation with adding activated carbon and without adding activated carbon was not significant in the mesophilic system. With addition of activated carbon, the archaea enriched in the fermentation liquid was mainly Methanosaeta concilii in the mesophilic system, whereas the archaea enriched in the fermentation liquid was mainly Methanosarcina acetivorans in the thermophilic system. The archaea enriched on activated carbon was mainly Methanosaeta concilii at mesophilic temperature, while the archaea enriched on activated carbon was mainly Methanosarcina thermophila at thermophilic temperature. |
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