| 包埋固定化活性污泥脱氮特性与微生物群落分析 |
| 摘要点击 2227 全文点击 1015 投稿时间：2016-11-03 修订日期：2016-11-30 |
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| 中文关键词 包埋固定化 深度处理 强化脱氮 微生物特性 高通量测序 |
| 英文关键词 embedding immobilization advanced treatment strengthened denitrification microbial properties high-throughput sequencing |
| 作者 | 单位 | E-mail | | 许晓毅 | 重庆大学城市建设与环境工程学院, 重庆 400045
重庆大学三峡库区生态环境教育部重点实验室, 重庆 400045 | xuxiaoyi@cqu.edu.cn | | 尤晓露 | 重庆大学城市建设与环境工程学院, 重庆 400045
重庆大学三峡库区生态环境教育部重点实验室, 重庆 400045 | | | 吕晨培 | 重庆大学城市建设与环境工程学院, 重庆 400045
重庆大学三峡库区生态环境教育部重点实验室, 重庆 400045 | | | 王斌 | 重庆大学城市建设与环境工程学院, 重庆 400045
重庆大学三峡库区生态环境教育部重点实验室, 重庆 400045 | | | 胡碧波 | 重庆大学城市建设与环境工程学院, 重庆 400045
重庆大学三峡库区生态环境教育部重点实验室, 重庆 400045 | |
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| 中文摘要 |
| 采用包埋固定化活性污泥法进行污水深度处理脱氮，针对不同初始总氮浓度的模拟废水，基于序批式间歇反应器的小试实验，探讨了包埋颗粒的脱氮效果及其微生物种群特性.结果表明，在包埋颗粒的体积投加率为10%，实验水温为10~15℃，DO为2~4 mg·L-1和初始COD浓度为80~100 mg·L-1条件下，不同初始总氮浓度（10~45 mg·L-1）和C/N比（1.78~10）的各反应器中，稳定期包埋颗粒的最大总氮去除负荷为7.78~23.18 mg·（L·h）-1.扫描电镜发现，包埋颗粒具有较好的孔隙结构，且颗粒内部与表面均存在微生物附着生长，已成为微生物的良好载体.高通量测序结果表明，较初始包埋污泥，包埋颗粒内部与表面的微生物群落构成发生了显著变化，包埋颗粒内微生物多样性良好，颗粒中脱氮菌属优势明显.包埋颗粒中存在异养硝化-好氧反硝化菌属，提升了包埋颗粒内非传统生物脱氮途径的潜能. |
| 英文摘要 |
| Immobilization of activated sludge was used to further remove nitrogen from secondary effluent. Intermittent sequencing batch reactor experiments were conducted to measure nitrogen removal in synthetic wastewater with initial total nitrogen concentrations (TN) of 10-45 mg·L-1 and C/N ratio of 1.78-10, and microbial community characteristic of embedding beads was investigated. When the packing ratio of embedding beads was 10%, and the temperature of wastewater, dissolved oxygen (DO), initial concentration of chemical oxygen demand (COD) were maintained at 10-15℃, 2-4 mg·L-1, and 80-100 mg·L-1, respectively, the results showed that the maximum total nitrogen removal loads ranged from 7.78 to 23.18 mg·(L·h)-1during the stable phase. SEM observations showed that the embedding beads were highly porous and microorganisms adhered to the interior and external surface of embedding beads, demonstrating that embedding beads acted as an ideal support material. Based on high-throughput sequencing analysis, the structure of microbial communities in the beads'interior and exterior changed significantly compared with embedding activated sludge. The advantage of denitrifying bacteria in embedding beads was obvious and the microbial diversity was good. Some microorganisms which can conduct both heterotrophic nitrification and aerobic denitrification, were identified. These processes may facilitate pathways for untraditional biological denitrification in the beads'interior. |
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