| 锰氧化菌Arthrobacter sp.HW-16的锰氧化特性和氧化机制 |
| 摘要点击 1414 全文点击 696 投稿时间：2016-11-12 修订日期：2016-12-05 |
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| 中文关键词 锰氧化细菌 锰氧化率 环境因子 活性因子 氧化机制 |
| 英文关键词 manganese-oxidizing bacterium Mn(Ⅱ) oxidation efficiency environmental factors Mn(Ⅱ) oxidizing active factor oxidation mechanism |
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| 中文摘要 |
| 锰作为一种常见的无机污染物，难以从环境中除去.本研究采用选择性培养基从锰矿土壤中分离到1株高效锰氧化细菌Arthrobacter sp.HW-16.此外，高通量测序发现，不同驯化条件下微生物群落结构有明显差异，且Arthrobacter在含Mn（Ⅱ）培养基里为优势菌属.本文对Arthrobacter sp.HW-16的微生物特性和锰氧化机制进行了初步的研究.结果表明，菌株HW-16的Mn（Ⅱ）耐受质量浓度高达5000 mg·L-1，菌株HW-16在3000 mg·L-1 Mn（Ⅱ）培养基里取得最大Mn（Ⅱ）氧化率为66.28%.单因素变量实验表明环境因子影响菌株HW-16的生长和Mn（Ⅱ）氧化率.菌株HW-16在30℃，pH 7.0，1%或3%盐度，200 r·min-1下生长得最好.高温（≥40℃），高pH（≥7），高转速和低盐度条件下Mn（Ⅱ）氧化率高.菌株HW-16通过合成锰氧化活性因子促使Mn（Ⅱ）氧化和产生碱性代谢产物促使Mn（Ⅱ）转化为沉淀. |
| 英文摘要 |
| Manganese is a common inorganic pollutant, which is difficult to remove from the environment. In this research, a high efficient manganese-oxidizing bacterium Arthrobacter sp. HW-16 was isolated from the manganese-rich soil using selective media. Besides, high-throughput sequencing revealed that there were significant differences of the microbial community compositions when bacteria were acclimated in different conditions, and Arthrobacte was the dominant genus in Mn(Ⅱ) containing media. In this paper, the microbiological properties of strain HW-16 and Mn(Ⅱ) oxidation mechanism were investigated. The results indicated that the maximal Mn(Ⅱ) tolerance mass concentration of strain HW-16 was 5000 mg·L-1,and it exhibited a decent Mn(Ⅱ) oxidation efficiency with the highest value of 66.28% at a Mn(Ⅱ) concentration of 3000 mg·L-1. Single factor experiments demonstrated that environmental factors could affect the growth and Mn(Ⅱ) oxidation efficiency of strain HW-16. At 30℃ or pH 7.0, at 1% or 3% salinity, and at 200 r·min-1, strain HW-16 got the highest biomass. While the highest Mn(Ⅱ) oxidation efficiency occurred at high temperature (≥40℃), high pH (≥7), high shaking speed and low salinity. Strain HW-16 could oxidize Mn(Ⅱ) by producing Mn(Ⅱ) oxidizing active factor and turn Mn(Ⅱ) into precipitation by synthesizing alkaline metabolites. |
