| 典型生活污水处理工艺对雌激素效应的去除 |
| 摘要点击 1906 全文点击 778 投稿时间：2016-07-31 修订日期：2016-09-22 |
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| 中文关键词 污水 酵母菌法 酶联免疫法 雌激素活性 雌二醇(E2) 去除率 |
| 英文关键词 wastewater yeast estrogen screen(YES) enzyme linked immunosorbent assay (ELSIA) estrogenicity E2 removal |
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| 中文摘要 |
| 针对水环境中内分泌干扰物低浓度、高风险的特点，采用酵母菌法和酶联免疫法对3个污水处理工艺流程中污水的雌激素活性和雌二醇（E2）水平进行评价.结果表明，污水厂进水的雌二醇当量（EEQ）和E2的水平分别为4.35~7.58 ng·L-1和36.95~83.43 ng·L-1.雌激素活性和E2的去除主要发生在生物处理阶段，如氧化沟、A2/O和A2/O+MBR工艺，且经以上工艺处理之后，雌激素活性和E2分别降低71.10%~75.54%和75.88%~80.72%.污泥对雌激素效应具有一定的去除作用，通过吸附作用污泥的EEQ和E2的含量分别为1.84~2.43 ng·g-1和8.45~12.84 ng·g-1.但污水厂出水仍具有较高的雌激素活性，其EEQ为1.06~2.19 ng·L-1，对受纳水体的水生生物具有潜在的危害.酶联免疫法与酵母菌法具有很好的一致性，并为水环境雌激素的快速筛选和评价提供了一个新方法. |
| 英文摘要 |
| Targeting at the characteristics of low concentration but high risk of endocrine disrupting compounds in the aquatic environment, the current study investigated the distribution of estrogenicity and E2 level using recombinant yeast estrogen screen (YES) and enzyme linked immunosorbent assay (ELSIA) during domestic wastewater treatment processes. The results showed that the levels of estrogenicity and E2 in influents of wastewater treatment plants were 4.35-7.58 ng·L-1 and 36.95-83.43 ng·L-1, respectively. It was found that biological treatment processes played a crucial role in the removal of estrogenicity and E2, such as oxidation ditch, A2/O and A2/O followed by MBR, where the removal of estrogenicity and E2 was 71.10%-75.54% and 75.88%-80.72%, respectively. The activated sludge, with an estrogencity level of 1.84-2.43 ng·g-1 and an E2 level of 8.45-12.84 ng·g-1, had the function of eliminating estrogenic effect to a certain extent by adsorption. The estrogenic activity in the effluent of domestic wastewater was 1.06-2.19 ng·L-1, which will pose potential risk to the aquatic life in the environment. Last but not least, ELISA had a good correlation with YES assay, which provides a new method for rapid screening and assessment of water environmental estrogen. |
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