| 厌氧氨氧化污泥群体感应信号分子检测及影响研究 |
| 摘要点击 1953 全文点击 1013 投稿时间：2016-07-04 修订日期：2016-10-17 |
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| 中文关键词 厌氧氨氧化 群体感应 酰基高丝氨酸内酯(AHL) 附着生长 菌群结构 |
| 英文关键词 anaerobic ammonium oxidation(ANAMMOX) quorum sensing N-acyl-homoserine lactone (AHL) adhesion growth microbial community structure |
| 作者 | 单位 | E-mail | | 陈舒涵 | 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875 | koalacsh@163.com | | 李安婕 | 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875 | liaj@bnu.edu.cn | | 王越兴 | 深圳市水务(集团)有限公司, 深圳 518001 | | | 吴玲娟 | 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875 | |
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| 中文摘要 |
| 厌氧氨氧化作为一种新型的脱氮工艺具有很好的应用前景，但厌氧氨氧化污泥富集培养困难.本研究以处理垃圾渗滤液的污泥作为接种污泥，在升流式厌氧污泥反应器（UASB）中启动厌氧氨氧化过程；检测驯化成功的厌氧氨氧化污泥中是否存在群体感应信号分子；并通过添加外源信号分子（cis-11-Methyl-2-dodecenoic acid，DSF和N-acyl-homoserine lactone，AHL），探究信号分子对厌氧氨氧化污泥附着生长能力的影响.结果表明，UASB反应器在运行150 d后，总氮去除率达到80%，菌群结构中厌氧氨氧化菌所占比例超过了20%，成功启动厌氧氨氧化工艺，且厌氧氨氧化污泥中存在低浓度的AHLs信号分子；人为添加3-碳位置取代基为羰基的AHLs（3-oxo-C6-HSL、3-oxo-C8-HSL、3-oxo-C10-HSL和3-oxo-C12-HSL）信号分子均能促进厌氧氨氧化污泥附着生长，而3-碳位置无取代基的AHLs中只有C12-HSL和C6-HSL存在积极影响，C8-HSL、C10-HSL和DSF信号分子对厌氧氨氧化污泥的附着生长能力并没有促进作用. |
| 英文摘要 |
| Anaerobic ammonium oxidation (ANAMMOX), an innovative nitrogen removal technology, has good prospects for applications. However, ANAMMOX bacteria grow slowly and are hardly accumulated in bioreactors. In this study, a UASB reactor inoculated with sludge from landfill leachate treatment plant was used for the start-up of ANAMMOX process. Besides, exogenous quorum sensing signals (DSF and AHL) were added to improve the adhesion of ANAMMOX sludge. The results showed that the UASB successfully started the ANAMMOX process within 150 days of operation. The total nitrogen removal rate reached 80% and the proportion of ANAMMOX bacteria rose to 20%. There was a low concentration of AHLs signal molecules in the ANAMMOX sludge. If the β-position substituent group of AHL added was a carbonyl group (including 3-oxo-C6-HSL, 3-oxo-C8-HSL, 3-oxo-C10-HSL and 3-oxo-C12-HSL), the adhesion growth ability of the ANAMMOX sludge could be improved. In the case of dosing with AHL molecules without β-position substituent groups, only C6-HSL and C12-HSL could promote the adhesion of ANAMMOX sludge. The additions of C8-HSL, C10-HSL and DSF all had negative effects on the adhesion of ANAMMOX sludge. |
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