| 再生水补水对河道底泥细菌群落组成与功能的影响 |
| 摘要点击 2768 全文点击 1027 投稿时间：2016-06-14 修订日期：2016-09-21 |
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| 中文关键词 再生水 城市河道 16S rRNA克隆文库 细菌组成 功能基因 T-RFLP |
| 英文关键词 reclaimed water urban river 16S rRNA library bacterial community functional genes T-RFLP |
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| 中文摘要 |
| 以北京市永定河麻峪湿地再生水补水河段河道底泥细菌群落为研究对象，联合限制性片段长度多态性（T-RFLP）技术，16S rRNA基因克隆文库技术和实时荧光定量qPCR技术，分析再生水补水口上游、补水口及补水口下游这3个断面的细菌群落组成与功能特征差异，尝试解释再生水补水对河道底泥细菌群落组成和功能特征的影响.结果表明，再生水中高浓度的碳氮磷含量是导致补水口细菌群落多样性显著升高和群落组成最为丰富的直接原因，人工湿地对碳氮磷浓度有较高的去除效率，净化后底泥细菌群落逐步恢复，表现出上下游相似的细菌群落多样性和结构组成；补水口处细菌群落的优势类群是变形菌门中β-Proteobacteria和δ-Proteobacteria，亚优势类群是Planctomycetes和Actinobacteria，而ε-Proteobacteria、Chloroflexi、Spirochaetes是补水口处的独有类群；氮碳磷循环是再生水补水河道底泥主要的生物地化循环过程，克隆文库中补水口以毛单胞菌属（Comamonas sp.）为优势类群的45.9%的克隆子与氮循环相关，其相对丰度高于上下游（27.7%和23.4%），以溶杆菌属（Lysobacter sp.）为优势类群的17.9%的克隆子与碳循环密切相关，其相对丰度高于上下游（14.4%和12.9%）；再生水中携带的痕量病原菌和抗生素等，在一定程度上改变了河道底泥细菌群落碳氮循环的转换方式，表现为补水口处固氮作用主要是红环菌属（Rhodocyclus sp.）通过光合作用实现，上下游以对植物具有促生作用的伯克氏菌属（Burkholderia sp.）为代表进行共生固氮.该研究结果为再生水补水河道人工湿地修复研究提供理论依据. |
| 英文摘要 |
| In order to study the effect of reclaimed water on bacterial community composition and function in urban river sediment, the changes of bacteria community diversity, composition and function in Mayu wetland upon the supply of reclaimed water were investigated by a range of sophisticated procedures, including Terminal Restriction Fragment Length Polymorphism(T-RFLP), 16S rRNA clone library technology, and Real-time Quantitative PCR Detecting System(qPCR).The results showed that carbon, nitrogen and phosphorus were major factors driving the variation of bacterial diversity and community structure in river sediment, and the bacteria were gradually recovered after purification in downstream under the effect of artificial wetland. In addition, the bacterial community in reclaimed water outfall was mainly constituted by β-Proteobacteria, δ-Proteobacteria, Bacteroidales and Cyanobacteriain, and ε-Proteobacteria, Chloroflexi and Spirochaetes were unique groups. Besides, the major biological geochemical cycle was nitrogen, carbon and phosphorus cycle in river sediment, which was closely related to functional genes. There were about 45.9% of the clones related to nitrogen cycle in reclaimed water outfall, such as Comamonas sp., higher than those of upstream and downstream (27.7% and 23.4%), 17.9% of the clones were closely related to the carbon cycle, such as Lysobacter sp., higher than those of upstream and downstream (14.4% and 12.9%). Furthermore, the trace of pathogenic bacteria and antibiotics in reclaimed water also changed the transformation pattern participating in carbon and nitrogen cycle, for example, Rhodocyclus sp. conducted nitrogen fixation by photosynthesis in reclaimed water outfall, whereas Burkholderia sp. fixes nitrogen by ways of plants symbiotic nitrogen fixation in upstream and downstream. This research provides theoretical reference for studies on remediation of reclaimed water supplying river by artificial wetland. |
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