| 沼泽红假单胞菌PSB06对辣椒根际微生物群落结构的影响 |
| 摘要点击 2212 全文点击 717 投稿时间：2016-06-11 修订日期：2016-09-05 |
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| 中文关键词 沼泽红假单胞菌PSB06 辣椒疫病 微生物多样性 微生物群落结构 微生态调控 |
| 英文关键词 Rhodopseudomonas palustris PSB06 phytophthora blight microbial diversity microbial community structure micro-ecological mechanism |
| 作者 | 单位 | E-mail | | 罗路云 | 湖南农业大学生物科学技术学院, 长沙 410128
湖南省农业科学院植物保护研究所, 园艺作物病虫害治理湖南省实验室, 长沙 410125 | luoluyun1992@163.com | | 金德才 | 中国科学院生态环境研究中心, 中国科学院环境生物技术重点实验室, 北京 100085 | | | 左晖 | 湖南生物机电职业技术学院, 长沙 410127 | | | 张卓 | 湖南省农业科学院植物保护研究所, 园艺作物病虫害治理湖南省实验室, 长沙 410125 | | | 谭新球 | 湖南省农业科学院植物保护研究所, 园艺作物病虫害治理湖南省实验室, 长沙 410125 | | | 张德咏 | 湖南省农业科学院植物保护研究所, 园艺作物病虫害治理湖南省实验室, 长沙 410125 | | | 卢向阳 | 湖南农业大学生物科学技术学院, 长沙 410128 | | | 刘勇 | 湖南省农业科学院植物保护研究所, 园艺作物病虫害治理湖南省实验室, 长沙 410125 | haoasliu@163.com |
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| 中文摘要 |
| 生物农药的使用极大地减少了对环境的污染，探究生物农药对致病菌的细菌多样性及群落分布将为后续研究生物农药对致病菌的微生态调控提供理论依据.本研究运用Illumina MiSeq高通量测序技术分析比较辣椒健康植株与疫病发病植株根际土壤微生物多样性，以及研究沼泽红假单胞菌PSB06对植株根际土壤的微生物多样性影响，探究沼泽红假单胞菌PSB06对辣椒疫病的微生态调控机制.结果显示，在第7 d和第14 d的来自同一处理的根际土壤细菌群落多样性变化没有显著差异，辣椒疫病发病植株根际土壤微生物多样性均小于健康植株根际土壤微生物多样性且喷洒沼泽红假单胞菌PSB06发酵液的土样微生物多样性最高；辣椒疫病发病植株根际土壤中放线菌丰度均小于健康植株且喷洒沼泽红假单胞菌菌剂PSB06的土壤中放线菌的丰度最高.辣椒发病植株与健康植株根际土壤中微生物多样性存在显著差异.施用沼泽红假单胞菌可以改善土壤微生物区系，提高土壤微生物群落丰富性以及土壤中放线菌所占的丰度. |
| 英文摘要 |
| The use of biological pesticide can greatly reduce the soil pollution in the environment. Exploring the effect of biological pesticide on community diversity and distribution of pathogenic bacteria will provide theoretic basis for subsequent researches on biological pesticide micro-ecological control. In order to explore the microbial ecological mechanism of pepper phytophthora blight, this research compared the difference of microbial diversity between rhizosphere soil of infected and healthy plants, and the effects of Rhodopseudomonas palustris PSB06 on microbial diversities of plant rhizosphere soil were investigated using Illumina MiSeq sequencing technology. The results showed that there was less difference in the microbial diversity from the same soil between the seventh day and the fourteenth day. The microbial diversity of rhizosphere soil of healthy plants was higher than that of rhizosphere soil of infected plants. The soil sprayed with Rhodopseudomonas palustris PSB06 exhibited the highest diversity. Moreover, the abundance of Actinomycetes in the rhizosphere soil of healthy plants was higher than that of infected plants, and the highest abundance of Actinomycetes was observed in the soil sprayed with Rhodopseudomonas palustris PSB06. The microbial diversity between rhizosphere soil of infected and healthy plants was significantly different. Spraying Rhodopseudomonas palustris PSB06 could significantly alter the microbial community structure of the soil. It could also increase the diversity of microorganism and the abundance of Actinomycetes in the soil. |
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