| 紫茉莉对石油污染盐碱土壤微生物群落与石油烃降解的影响 |
| 摘要点击 3176 全文点击 1785 投稿时间：2015-03-23 修订日期：2015-05-11 |
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| 中文关键词 石油污染盐碱土壤 石油烃 微生物群落 磷脂脂肪酸 紫茉莉 |
| 英文关键词 petroleum-contaminated saline-alkali soil petroleum hydrocarbon microbial communities phospholipid fatty acids(PLFAs) Mirabilis jalapa Linn. |
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| 中文摘要 |
| 以石油污染盐碱土壤为研究对象,利用磷脂脂肪酸(PLFAs)活性微生物标记法,分析紫茉莉(Mirabilis jalapa Linn.)根际土壤微生物群落结构的动态变化,探讨紫茉莉生长对根际土壤微生物与石油烃(TPH)降解的影响. 结果表明,供试土壤中先后出现了22种微生物PLFAs,包括标识细菌的饱和脂肪酸(SAT)、标识革兰氏阳性菌(G+)的末端支链型饱和脂肪酸(TBSAT)、标识革兰氏阴性菌(G-)的单不饱和脂肪酸(MONO)和环丙脂肪酸(CYCLO)、标识真菌的多不饱和脂肪酸(PUFA)和标识放线菌的中间型支链型饱和脂肪酸(MBSAT)等六大类型. 与未种紫茉莉土壤(CK)相比,根际土壤微生物PLFAs种类变异率在春、夏、秋季分别为71.4%、69.2%和33.3%; TPH降解率在春、夏、秋季分别提高了47.6%、28.3%、18.9%. 相关性分析表明,石油烃的降解在CK土壤中与77.8%的PLFAs具有正相关关系(r>0),55.6%的种类具有高度正相关关系(r≥0.8),其中,与SAT和MONO类群的相对含量正相关,相关系数分别为0.92、0.60; 根际土壤中仅与42.1%的PLFAs正相关,21.1%的种类高度正相关,与TBSAT、MONO和CYCLO类群的相对含量正相关,相关系数分别为0.56、0.50、0.07. 说明紫茉莉生长对根际土壤微生物群落结构及TPH降解速率均具有较大影响,且随生长季节的不同而有很大差异. 该结果将为开展石油污染盐碱土壤的植物修复技术研究提供理论依据. |
| 英文摘要 |
| In order to explore the effect of Mirabilis jalapa Linn. growth on the structure characteristics of the microbial community and the degradation of petroleum hydrocarbon (TPH) in the petroleum-contaminated saline-alkali soil, Microbial biomass and species in the rhizosphere soils of Mirabilis jalapa Linn. in the contaminated saline soil were studied with the technology of phospholipid fatty acids(PLFAs) analysis. The results showed that comparing to CK soils without Mirabilis jalapa Linn., the ratio of PLFAs species varied were 71.4%, 69.2% and 33.3% in the spring, summer and autumn season, respectively. In addition, there was distinct difference of the biomasses of the microbial community between the CK and rhizosphere soils and among the difference seasons of growth of Mirabilis jalapa Linn.. Compare to CK soil, the degradation rates of total petroleum hydrocarbon (TPH) was increased by 47.6%, 28.3%, and 18.9% in spring, summer, and autumn rhizosphere soils, respectively. Correlation analysis was used to determine the correlation between TPH degradation and the soil microbial community. 77.8% of the total soil microbial PLFAs species showed positive correlation to the TPH degradation (the correlation coefficient r>0), among which, 55.6% of PLFAs species showed high positive correlation(the correlation coefficient was r≥0.8). In addition, the relative content of SAT and MONO had high correlation with TPH degradation in the CK sample soils,the correlation coefficient were 0.92 and 0.60 respectively; However, the percent of positive correlation was 42.1% in the rhizosphere soils with 21.1% of them had high positive correlation. The relative content of TBSAT, MONO and CYCLO had moderate or low correlation in rhizosphere soils, and the correlation coefficient were 0.56, 0.50, and 0.07 respectively. Our study showed that the growth of mirabilis Mirabilis jalapa Linn. had a higher influence on the species and biomass of microbial community in the rhizosphere soils, and the results will provide a basis theory for the research of phytoremediation petroleum contaminated saline soil. |
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