| 氮素添加对樟树林红壤微生物的影响 |
| 摘要点击 4425 全文点击 1989 投稿时间：2012-11-25 修订日期：2013-01-25 |
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| 中文关键词 氮添加 红壤 微生物量C、N 微生物多样性 BIOLOG |
| 英文关键词 nitrogen addition red soil microbial biomass C, N microbial diversity BIOLOG |
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| 中文摘要 |
| 为了研究氮素添加对红壤微生物的影响,以湖南植物园樟树红壤林地为研究区,设定了氮素添加的3个水平,即对照N0:0 g·m-2、低氮N1: 5 g·m-2和高氮N2: 15 g·m-2,利用平板计数法,氯仿熏蒸法和BIOLOG微平板技术,分析了氮素添加1个月和13个月后红壤微生物数量、生物量C、N以及群落功能多样性的响应变化. 结果表明,氮素添加1个月后土壤细菌数量显著增加,13个月后显著减少,真菌和放线菌数量变化不明显; 氮素添加1个月后,微生物量C、N均随氮素的增加而增加,氮素添加13个月后土壤微生物量C与1个月相比显著增加,土壤微生物量N却有降低的趋势,但差异不显著; 土壤微生物的碳源利用率因施氮处理而发生分异. Shannon、Simpson和McIntosh指数的结果显示AWCD在氮素添加不同水平和不同时期有一定的差异,但差异并不显著. |
| 英文摘要 |
| In order to investigate the effects of nitrogen addition on the red soil microbial communities in Cinnamomum camphora plantation, three treatments of nitrogen addition were designated as control (N0: 0 g·m-2), low nitrogen (N1: 5g·m-2) and high nitrogen (N2:15g·m-2). Soil microbial numbers, microbial biomass carbon (C), biomass N and microbial community functional diversity were analyzed using the methods of plate counting, chloroform fumigation and BIOLOG system, respectively. The results showed that the numbers of bacteria in N1 and N2 were significantly higher than the control 1 month after nitrogen addition, but significantly lower than the control 13 months after nitrogen addition, and the number of fungi and actinomycetes were not significantly changed after nitrogen addition. The soil microbial biomass C, N increased with the increase of nitrogen at 1 month, but the soil microbial biomass C increased significantly 13 months after nitrogen addition when compared with 1 month after nitrogen addition. The soil microbial biomass N was lower 13 months after nitrogen addition when compared with 1 month after nitrogen addition, but the difference was not significant (P＞0.05). The variation of the carbon utilization efficiency of soil microbial communities was resulted from the nitrogen addition. The indices of Shannon index, Simpson index and McIntosh index were calculated to show the differences in nitrogen treatments and in times, which turned out to be insignificant. |
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