| 给水管网中耐氯分枝杆菌的灭活特性及机制研究 |
| 摘要点击 4425 全文点击 1701 投稿时间：2012-04-11 修订日期：2012-06-24 |
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| 中文关键词 产黏液分枝杆菌 消毒特性 细胞表面疏水率 给水管网 耐氯机制 |
| 英文关键词 Mycobacteria mucogenicum inactivation characteristics bacteria surface hydrophobic drinking water distribution system chlorine resistance mechanism |
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| 中文摘要 |
| 近年来,在饮用水管网中检测到耐受消毒剂的微生物,对于饮用水安全造成威胁.课题组由南方某城市自来水管网中分离出1株耐氯的产黏液分枝杆菌(Mycobacteria mucogenicum),并对其灭活特性和耐氯机制进行了研究.使用自由氯、一氯胺和二氧化氯对其进行消毒实验,测定CT值. 99.9%灭活产黏液分枝杆菌时,自由氯的CT值为(76.25±47.55)mg·min·L-1,一氯胺为(1396±382) mg·min·L-1,二氧化氯为(13.5±4.9)mg·min·L-1.采用透射电镜对产黏液分枝杆菌的消毒过程进行观察,发现消毒后细菌结构疏松,细胞器层次变得不清晰,核心肿胀溶解.产黏液分枝杆菌具有较好的疏水性,测定其表面疏水率为37.2%,远高于其他细菌,使得亲水性消毒剂不易进入细菌内部,是其耐受含氯消毒剂的原因之一. |
| 英文摘要 |
| In recent years, chlorine-resistant bacteria were detected in drinking water distribution systems which threatened the drinking water safety. Our group detected one strain named Mycobacteria mucogenicum from the drinking water distribution system of a city in south China. This paper studied chlorine resistance and mechanism of Mycobacteria mucogenicum. Inactivation experiments of one strain Mycobacteria mucogenicum were conducted with free chlorine, monochloramine and chlorine dioxide. The CT values of 99.9% inactivation by free chlorine, monochloramine and chlorine dioxide were detected as (76.25±47.55)mg·min·L-1, (1396±382)mg·min·L-1, (13.5±4.9) mg·min·L-1. Using transmission electronmicroscopy (TEM) observed the inactivation process of Mycobacteria mucogenicum. The bacteria surface hydrophobic of Mycobacteria mucogenicum was 37.2%. Mycobacteria mucogenicum has a higher hydrophobicity than other bacteria which prevented the diffusion of chlorine into cells. Mycobacteria mucogenicum is more resistant to chorine than other bacteria. |
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