| 分子印迹-化学发光分析法测定水中苯酚的研究 |
| 摘要点击 6687 全文点击 1759 投稿时间：2010-01-31 修订日期：2010-03-23 |
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| 中文关键词 分子印迹聚合微球 悬浮聚合 苯酚 化学发光 鲁米诺 高锰酸钾 |
| 英文关键词 molecularly imprinted polymeric microspheres(MIPM) suspension polymerization phenol |
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| 中文摘要 |
| 以丙烯酰胺为功能单体，季戊四醇三丙烯酸酯为交联剂，偶氮二异丁腈为引发剂，乙腈为稀释剂，采用悬浮聚合法，在水性体系中制备了表面带孔的MIPM, MIPM吸附平衡时间为120 min，饱和吸附量分别为7.03 μmol·L-1.以邻苯二酚和对苯二酚为竞争分子，分离因子分别达到1.66和1.99. 本研究制备的MIPM具有良好的选择吸附性能和良好的再生性能.以MIPM为分子识别物，利用鲁米诺-高锰酸钾化学发光体系结合流动注射技术，建立了测定苯酚的分子印迹-化学发光分析方法.测得苯酚溶液浓度在1×10-3～100 μg·L-1线性范围内线性相关，相关系数r为0.9996，相对标准偏差为0.99%，检出限为9×10-4 μg·L-1，用该方法测定水样中苯酚的加标回收率为99%～105%,结果较好. |
| 英文摘要 |
| Using phenol as template, acrylamide as monomers, pentaerythritol triacrylate as crosslinking agent, azodiisobutyronitrile as initiating agent, acetomitrile as thinner, we synthesized MIPM in aquosity system with suspension polymerization,on which surface there are some holes. The adsorbance of MIPM to phenol will no longer increased after two hours, it means that it reaches the saturation which were 7.03 μmol·L-1. The separate genes of MIPM were 1.66 and 1.99 with pyrocatechol and hydroquinone as competitors. The MIPM had the better effect of adsorption and selectivity. Using MIPM as molecule recognizates, we set up a new method which was molecularly imprinted technique-flow injection- chemoluminescence analysis to detect phenol. The result shows that the range of phenol concentration from 1×10-3 μg·L-1 to 100 μg·L-1 with adsorbed by MIPM is linearly dependent, which relativity coefficient is 0.9996 and the relative standard deviation is 0.99%, the detection limit is 9×10-4μg·L-1. Using the method to detecting phenol from the water samples, the recoveries of phenol is 99%-105%, the result is pretty good. |
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