| 降解喹啉的假单胞菌BW003菌株的分离、鉴定和降解特性 |
| 摘要点击 4038 全文点击 2147 投稿时间：2007-12-11 修订日期：2008-03-03 |
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| 中文关键词 Pseudomonas sp. 喹啉 微生物降解 代谢产物 |
| 英文关键词 Pseudomonas sp. quinoline biodegradation metabolic products |
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| 中文摘要 |
| 从焦化废水活性污泥中分离出1株能利用喹啉作为唯一碳、氮源及能源的高效降解菌，16S rRNA鉴定为Pseudomonas sp..降解试验表明，菌株能将192～911 mg/L的喹啉在3～8 h内有效地降解，去除率均在96％～98％.最适降解温度为30℃，最适降解初始pH值为8.0.跟踪分析降解中间产物发现：在整个降解过程中，至少有43％的喹啉转化为2-羟基喹啉，其后有0.69％的2-羟基喹啉转化为2,8-二羟基喹啉，随后再转化为8-羟基香豆素；另外，至少有48％的喹啉中氮直接转化为氨氮，且外加碳源能促进氨氮的进一步转化，控制好碳氮比将能有效去除喹啉及其转化的各种污染物. |
| 英文摘要 |
| A bacterial strain BW003，which could utilize quinoline as sole carbon，nitrogen and energy source，was isolated from the activated sludge in a coking wastewater treatment plant.According to the 16S rRNA gene sequence analysis，the strain was identified as Pseudomonas sp..Biodegradation experiments showed that the strain could degrade 192-911 mg/L of quinoline efficiently within 3-8 h，and the removal rates of quinoline were ranged from 96％ to 98%.The optimum conditions for the degradation were 30°C and pH 8.Metabolic products analysis revealed that at least 43% quinoline was first transformed into 2-hydroxyquinoline，0.69% 2-hydroxyquinoline was then transformed into 2,8-dihydroxyquinoline，and subsequently into 8-hydroxycoumarin in the process of biodegradation.Additionally，at least 48% of nitrogen in quinoline was transformed into NH3-N directly and external carbon source could promote the nitrogen transformation，demonstrating that the quinoline and its metabolic products could be eliminated if controlling proper C/N ratio. |
