| 脱氮硫杆菌的分离鉴定和反硝化特性研究 |
| 摘要点击 4358 全文点击 2650 投稿时间：2007-10-20 修订日期：2007-12-11 |
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| 中文关键词 自养反硝化 脱氮硫杆菌 生理生化特性 16S rDNA 反硝化特性 硝酸盐氮 盐度 毒性 |
| 英文关键词 autotrophic denitrification Thiobacillus denitrificans biochemical and physiological characters 16S rDNA denitrification characteristic nitrate salinity toxicity |
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| 中文摘要 |
| 从土壤中分离到1株高活性自养反硝化菌TD, 并对其进行了鉴定和硝酸盐还原特性研究.该菌株为革兰氏阴性短杆菌,严格自养.16S rDNA 序列分析表明,该菌株与 Thiobacillus denitrificans 相似性为99.85%.结合生理生化特性和16S rDNA序列分析,确定菌株TD为脱氮硫杆菌.通过对该菌的生长特性和反硝化特性的研究表明，该菌在初始pH为6.85，32.8℃ 培养条件下脱氮效果最佳；在初始pH为6.90，29.5℃培养条件下生长最快.该菌生长比较缓慢，没有明显的稳定期，对数生长期阶段的反硝化能力最强，反硝化速率最快，达到了2.245 mg·(L·h)-1，在培养过程中培养基pH值明显下降.较高盐度对该菌株的反硝化活性有抑制作用.该菌株的急性毒性实验结果显示，脱氮硫杆菌对健康鱼体几乎无毒，属于无毒性菌株. |
| 英文摘要 |
| A autotrophic denitrifying bacterial strain, TD, was isolated from soil and the strain was identified and characterized. The strain was gram negative, strictly chemolithoautotrophic, and short rod shaped bacterium. The 16S rDNA sequence analysis revealed that strain TD had a similarity of 99.85% with Thiobacillus denitrificans. According to the morphologic, physiobiochemical characteristics and the analysis of its 16S rDNA, the strain was identified as Thiobacillus denitrificans. Studies showed that the optimal conditions for denitrification were pH 6.85 and 32.8℃, while the optimal growth conditions were pH 6.90 and 29.5℃. The bacteria grew slowly with no apparent stable phase. The maximal denitrification rate reached 2.245 mg·(L·h)-1, which was found in exponential phase. In the process of the culture, the medium pH decreased significantly. Relatively high salinity restrained the denitrification activity of Thiobacillus denitrificans. The acute toxicity test results showed that Thiobacillus denitrificans was non-toxic. |
