| 饮用水中几种细菌计数方法的比较 |
| 摘要点击 3003 全文点击 4120 投稿时间：2003-08-07 修订日期：2003-09-25 |
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| 中文关键词 饮用水 直接计数 平板计数 活细菌直接计数 细菌 |
| 英文关键词 drinking water enumeration PC DVC bacteria |
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| 中文摘要 |
| 比较采用不同培养基的平板计数(Plate Counts,PC)方法,以及不同荧光染色剂的显微镜直接计数方法与常规计数方法的差别.研究认为,常规平板计数方法并不能准确反映饮用水中实际存活的细菌数量;吖啶橙直接计数(Acridine Orange Direct Counts,AODC)的结果最高,较常规平板计数方法高出3～4个量级;活细菌直接计数(Direct Viable Counts,DVC)中,DVC-N.A.、DVC-CTC和DVC-BacLight等计数方法的结果较常规平板计数结果高出2～3个量级.以地表水为水源的水厂出水中活细菌数占总细菌数的比例在10%左右.
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| 英文摘要 |
| Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts(AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%. |
