| 民勤荒漠绿洲过渡带人工梭梭林土壤细菌群落结构及功能预测 |
| 摘要点击 677 全文点击 225 投稿时间：2023-02-20 修订日期：2023-04-14 |
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| 中文关键词 土壤细菌 群落结构 PICRUSt2功能预测 人工梭梭林 荒漠绿洲过渡带 |
| 英文关键词 soil bacteria community structure PICRUSt2 function prediction artificial Haloxylon ammodendron forest desert-oasis ecotone |
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| 中文摘要 |
| 通过探究人工梭梭林栽植对荒漠土壤细菌群落结构及功能的影响,为荒漠绿洲过渡带土壤微生态修复和土地质量改善提供数据参考.采用Illumina高通量测序技术和PICRUSt2功能预测分析方法,分析土壤细菌群落结构及功能,并用Mantel test和冗余分析（RDA）解释影响土壤细菌群落结构及功能的理化因子.结果表明：①梭梭林土壤细菌OTU数、Chao1指数和Shannon指数均显著高于流动沙丘土壤,且PCoA分析和Adonis检验发现梭梭林与流动沙丘土壤细菌群落结构差异显著（P=0.001）.②所有样品共检测到细菌34门、89纲、174目、262科和432属,变形菌门、放线菌门、蓝藻菌门和绿弯菌门占土壤细菌相对丰度的76.05%,属于土壤优势细菌,其中梭梭林土壤放线菌门相对丰度极显著高于流动沙丘土壤（P<0.01）.③PICRUSt2功能预测发现,梭梭林土壤细菌群落包括6类一级功能和28类二级功能,其中碳水化合物的代谢、氨基酸代谢、辅助因子和维生素的代谢相对丰度均大于10%,是细菌主要代谢功能.④梭梭林的栽植显著改善土壤有机质等养分含量.土壤pH、有机质、全氮和速效磷为影响细菌群落的主要理化因子,其中土壤有机质显著影响土壤细菌群落结构（P<0.05）及代谢功能（P<0.05）.综上,人工梭梭林有助于增加荒漠土壤微生物多样性,可提高土壤细菌代谢功能基因相对丰度,改善荒漠土壤微环境. |
| 英文摘要 |
| Exploring the effects of artificial Haloxylon ammodendron forest planting on the structure and function of a desert soil bacterial community provides data reference for soil micro-ecological restoration and land quality improvement in desert oasis transition zones. Illumina high-throughput sequencing technology and PICRUSt2 functional prediction analysis were used to identify and analyze the structure and function of soil bacterial communities, and the Mantel correlation test and RDA analysis were used to explain the physicochemical factors affecting the structure and function of soil bacterial communities. The results showed that:① the soil bacterial OTU number, Chao1 index, and Shannon index were significantly higher in the H. ammodendron forest than in the mobile dune soil, and the PCoA analysis and Adonis test showed significant differences in the soil bacterial community structure between H. ammodendron and mobile dune soil (P=0.001). ② A total of 34 phyla, 89 classes, 174 orders, 262 families, and 432 genera of bacteria were detected in all samples, and the phyla Proteobacteria, Actinobacteria, Cyanobacteria, and Chloroflexi accounted for 76.05% of the relative abundance of soil bacteria, which belonged to the dominant soil bacteria, among which the relative abundance of Actinobacteria in H. ammodendron forest soil was extremely significantly higher than that in mobile dune soil (P < 0.01). ③PICRUSt2 function prediction revealed that the soil bacterial community of H. ammodendron forest included six categories of primary functions and 28 categories of secondary functions, among which the metabolism of carbohydrates, metabolism of amino acids, and metabolism of cofactors and vitamins were all greater than 10% in relative abundance and were the main metabolic functions of H. ammodendron forest soil bacteria. ④ The planting of H. ammodendron forest significantly improved the nutrient content of soil organic matter and other nutrients. Soil pH, organic matter, total nitrogen, and fast-acting phosphorus were the main physicochemical factors affecting the bacterial community, with soil organic matter significantly affecting the soil bacterial community structure (P < 0.05) and metabolic function (P < 0.05). In conclusion, the artificial H. ammodendron forest helped to increase desert soil microbial diversity, increase the relative abundance of soil bacterial metabolic function genes, and improve the desert soil microenvironment. |
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