| 高效异养硝化-好氧反硝化菌Glutamicibacter sp.WS1低温下对多种氮源的脱氮特性及氮代谢机制 |
| 摘要点击 1138 全文点击 386 投稿时间：2022-10-08 修订日期：2022-11-16 |
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| 中文关键词 耐低温 异养硝化-好氧反硝化 Glutamicibacter sp.WS1 脱氮特性 氮代谢路径 |
| 英文关键词 cold-tolerant heterotrophic nitrification-aerobic denitrification Glutamicibacter sp. WS1 nitrogen removal characteristics nitrogen metabolism pathway |
| 作者 | 单位 | E-mail | | 魏渤惠 | 河北科技大学建筑工程学院, 石家庄 050018 | w15630468932@163.com | | 罗晓 | 河北科技大学环境科学与工程学院, 河北省污染防治生物技术实验室, 石家庄 050018 | | | 吕鹏翼 | 河北科技大学环境科学与工程学院, 河北省污染防治生物技术实验室, 石家庄 050018 | lvpeng.yi@163.com | | 马文凯 | 河北科技大学建筑工程学院, 石家庄 050018 | | | 苏金卫 | 河北科技大学环境科学与工程学院, 河北省污染防治生物技术实验室, 石家庄 050018 | | | 李伟 | 河北科技大学环境科学与工程学院, 河北省污染防治生物技术实验室, 石家庄 050018 | | | 崔建升 | 河北科技大学环境科学与工程学院, 河北省污染防治生物技术实验室, 石家庄 050018 | |
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| 中文摘要 |
| 针对污水处理厂冬季生物脱氮效率低、出水水质不达标的问题，从活性污泥中分离出1株耐低温异养硝化-好氧反硝化菌株Glutamicibacter sp.WS1.采用PCR技术扩增该菌株的脱氮功能基因，研究其对不同氮源的低温脱氮效能，通过单因素实验探究环境因子对其低温好氧反硝化性能的影响，并利用氮平衡解析其氮代谢路径.结果表明，菌株WS1含有氮代谢相关的功能基因amoA、napA、nirS和nirK；在15℃低温条件下，菌株WS1在以NH4+-N、NO3--N、NO2--N+NO3--N和NH4+-N+NO3--N为氮源时，对各无机氮的去除率分别为100%、98.10%、99.87%+100%和100%+94.92%；菌株WS1的最佳反硝化条件：柠檬酸钠为碳源、C/N为16、pH为8、ρ（DO）为4.5~6.8 mg ·L-1和温度为30℃；在低温（15℃）和低C/N （10）条件下，菌株WS1对NO3--N的去除率达到92.50%；异养硝化-好氧反硝化/好氧反硝化和同化作用是菌株WS1去除不同氮源底物的主要途径，其中大部分的无机氮（47%~56%）通过异养硝化-好氧反硝化/好氧反硝化作用转化为了气态氮.菌株WS1在低温污水脱氮领域具有广阔的应用前景. |
| 英文摘要 |
| For resolving the problems of poor nitrogen removal efficiency and substandard effluent quality in wastewater treatment plants during winter, a cold-tolerant strain Glutamicibacter sp. WS1, with heterotrophic nitrification-aerobic denitrification ability, was isolated from activated sludge. The functional genes for nitrogen conversion of strain WS1 were amplified by PCR, and the nitrogen removal characteristics of the strain were verified under different nitrogen sources at 15℃. In addition, the effects of environmental factors on the aerobic denitrification performance of the strain were explored at low temperature. Finally, a reasonable nitrogen metabolism pathway of strain WS1 was resolved based on functional genes and nitrogen balance analysis. The results showed that strain WS1 contained functional genes related to nitrogen conversion, including amoA, napA, nirS, and nirK genes. Notably, nirS and nirK genes coexisted in the strain. At the low temperature of 15℃, with NH4+-N, NO3--N, NO2--N+NO3--N, and NH4+-N+NO3--N as nitrogen sources, the corresponding removal efficiencies of strain WS1 were 100%, 98.10%, 99.87%+100%, and 100%+94.92%, respectively. The optimal denitrification performance of the strain was achieved with sodium citrate as the carbon source, C/N of 16, pH of 8, DO of 4.5-6.8 mg·L-1, and temperature of 30℃. In addition, the NO3--N removal efficiency of strain WS1 reached 92.50% under low temperature (15℃) and low C/N (10) conditions. Based on the results of PCR amplification and nitrogen balance analysis, heterotrophic nitrification-aerobic denitrification/aerobic denitrification and assimilation were the main pathways for nitrogen substrate removal by strain WS1, in which most of the inorganic nitrogen (47%-56%) was converted to gaseous nitrogen through heterotrophic nitrification-aerobic denitrification/aerobic denitrification. Strain WS1 has broad application prospects in the treatment of low-temperature nitrogenous wastewater. |
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