| 外源褪黑素对锑胁迫下水稻幼苗生长和抗氧化系统的影响 |
| 摘要点击 1345 全文点击 450 投稿时间：2022-05-27 修订日期：2022-07-05 |
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| 中文关键词 锑胁迫 水稻 褪黑素(MT) 抗氧化系统 AsA-GSH循环 |
| 英文关键词 antimony stress rice melatonin(MT) antioxidant system AsA-GSH cycle |
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| 中文摘要 |
| 为了探讨外源添加褪黑素（MT）对锑（Sb）胁迫下水稻幼苗影响，以华润2号水稻幼苗为研究对象进行水培实验，采用荧光探针定位技术对水稻幼苗根尖的活性氧（ROS）进行荧光定位，并对水稻幼苗根部根系活力、丙二醛（MDA）含量、ROS （H2 O2、O2- ·）含量、抗氧化酶（SOD、POD、CAT、APX）活性和抗氧化剂（GSH、GSSG、AsA、DHA）含量进行分析.结果表明，外源添加MT能缓解Sb胁迫对水稻幼苗生长的不利影响，提高水稻幼苗的生物量；与单独Sb处理相比，添加100 μmol ·L-1的MT使水稻根系活力和总根长分别提高44.1%和34.7%，MDA、H2 O2和O2- ·含量分别降低37.0%、32.7%和40.5%.此外，MT处理不仅使POD和CAT活性分别提高54.1%和21.8%，还能调节AsA-GSH循环.由此可见，外源添加100 μmol ·L-1 MT可以促进水稻幼苗生长，提高水稻幼苗抗氧化能力，缓解Sb胁迫对水稻的脂质过氧化损害，从而提高水稻幼苗在Sb胁迫下的抗逆性. |
| 英文摘要 |
| To investigate the effect of exogenous application of melatonin (MT) on rice seedlings under antimony (Sb) stress, hydroponic experiments were carried out with rice seedlings (Huarun No.2). The fluorescent probe localization technology was used to locate the reactive oxygen species (ROS) in the root tips of rice seedlings, and the root viability, malondialdehyde (MDA) content, ROS (H2O2 and O2-·) content, antioxidant enzyme (SOD, POD, CAT, and APX) activities, and antioxidant (GSH, GSSG, AsA, and DHA) contents in the roots of rice seedlings were analyzed. The results showed that exogenous addition of MT could alleviate the adverse effects of Sb stress on the growth and increase the biomass of rice seedlings. Compared with the Sb treatment, the application of 100 μmol·L-1 MT increased rice root viability and total root length by 44.1% and 34.7% and reduced the content of MDA, H2O2, and O2-· by 30.0%, 32.7%, and 40.5%, respectively. Further, the MT treatment increased the activities of POD and CAT by 54.1% and 21.8%, respectively, and also regulated the AsA-GSH cycle. This research indicated that exogenous application of 100 μmol·L-1MT can promote the growth and antioxidant ability of rice seedlings and alleviate the damage of lipid peroxidation by Sb stress, thus improving the resistance of rice seedlings under Sb stress. |
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