| 磷石膏和碱蓬对盐渍化土壤水盐及细菌群落结构的影响 |
| 摘要点击 1270 全文点击 481 投稿时间：2022-04-19 修订日期：2022-06-22 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 磷石膏 碱蓬 细菌群落结构多样性 盐碱地改良 高通量测序 |
| 英文关键词 phosphogypsum Suaeda salsa diversity of bacterial community structure saline-alkali soil improvement high-throughput sequencing |
|
| 中文摘要 |
| 盐渍土改良是农田土壤环境不可忽视的重要问题，土壤盐分的改变势必会影响土壤细菌群落.试验基于河套灌区中度盐渍土，以当地无改良措施枸杞园为对照（CK），设施加磷石膏（LSG）、枸杞间种碱蓬（JP）及施加磷石膏和枸杞间种碱蓬（LSG+JP）的改良处理，探究枸杞生育期内不同改良方式对土壤水分、盐分、养分和细菌群落结构多样性的影响.结果表明，相较CK，在开花期至落叶期LSG+JP显著降低了土壤EC值和pH值（P<0.05），平均降低39.96%和7.25%；全生育期内LSG+JP显著提高了土壤有机质（OM）和速效磷（AP）含量（P<0.05），年平均提高81.85%和203.50%；开花期和落叶期LSG+JP显著提高了全氮（TN）含量（P<0.05），年平均提高48.91%.改良初期LSG+JP的Shannon指数较CK提高了3.31%和6.54%，Chao1指数较CK提高了24.95%和43.26%；土壤优势菌门为变形菌门、拟杆菌门、放线菌门和酸杆菌门，优势菌属为鞘脂单胞菌属.开花期至落叶期改良处理的变形菌门相对丰度较CK增加了0.50%~16.27%，改良处理的放线菌门相对丰度在开花期和盛果期较CK增加了1.91%~4.98%.冗余分析（RDA）结果表明pH值、含水率（WT）和AP是影响细菌群落组成的重要因素，相关性热图显示变形菌门和拟杆菌门与EC值呈极显著负相关（P<0.001），放线菌门和硝化螺菌与EC值呈显著负相关（P<0.01）.综上，施用磷石膏和枸杞间种碱蓬（LSG+JP）能显著降低土壤盐分、提高养分和改善土壤细菌群落结构多样性，有利于河套灌区盐渍土长效改良和维护土壤生态健康. |
| 英文摘要 |
| The improvement of saline soil is an important issue that cannot be ignored in the farmland soil environment. The change in soil salinity will inevitably affect the soil bacterial community. This experiment was based on moderately saline soil in the Hetao Irrigation Area, conducted by applying phosphogypsum (LSG), interplanting Suaeda salsa with Lycium barbarum (JP) and applying phosphogypsum and interplanting S. salsa with L. barbarum (LSG+JP),and the local unimproved soil of a L. barbarum orchard was used as the control (CK), to explore the effects of different improvement methods on soil moisture, salinity, nutrients, and bacterial community structure diversity during the growth period of L. barbarum. The results showed that compared with that under CK, the LSG+JP treatment significantly decreased the soil EC value and pH value from the flowering stage to the deciduous stage (P<0.05), with an average decrease of 39.96% and 7.25%, respectively; the LSG+JP treatment significantly increased soil organic matter (OM) and available phosphorus (AP) content during the whole growth period (P<0.05), with an average annual increase of 81.85% and 203.50%, respectively. The total nitrogen (TN) content was significantly increased in the flowering and deciduous stages (P<0.05), with an annual average increase of 48.91%. The Shannon index of LSG+JP in the early stage of improvement was increased by 3.31% and 6.54% compared with that of CK, and the Chao1 index was increased by 24.95% and 43.26% compared with that of CK, respectively. The dominant bacteria in the soil were Proteobacteria, Bacteroidetes, Actinobacteria, and Acidobacteria, and the dominant genus was Sphingomonas. Compared with that in CK, the relative abundance of Proteobacteria in the improved treatment increased by 0.50%-16.27% from the flowering stage to the deciduous stage, and the relative abundance of Actinobacteria in the improved treatment increased by 1.91%-4.98% compared with that in CK in the flowering and full-fruit stages. Redundancy analysis (RDA) results showed that pH, water content (WT), and AP were important factors affecting bacterial community composition, and the correlation heatmap showed that Proteobacteria, Bacteroidetes, and EC values were significantly negatively correlated (P<0.001); Actinobacteria and Nitrospirillum were significantly negatively correlated with EC values (P<0.01). In conclusion, the application of phosphogypsum and interplanting S. salsa with L. barbarum (LSG+JP) could significantly reduce soil salinity, increase nutrients, and improve the diversity of soil bacterial community structure, which is beneficial to the long-term improvement of saline soil in the Hetao Irrigation Area and the maintenance of soil ecological health. |
|
|
|
