| 温度对ANAMMOX生物膜工艺的脱氮影响与菌群结构分析 |
| 摘要点击 1901 全文点击 681 投稿时间：2021-05-27 修订日期：2021-06-28 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 温度 改性聚氨酯泡沫塑料填料 无纺布填料 微生物群落 |
| 英文关键词 anaerobic ammonium oxidation(ANAMMOX) temperature modified polyurethane foam carrier non-woven fabric carrier microbial community |
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| 中文摘要 |
| 针对厌氧氨氧化（anaerobic ammonium oxidation，ANAMMOX）工艺常低温运行问题，分别采用无纺布与改性聚氨酯泡沫塑料填料序批式生物膜反应器（sequence batch biofilm reactor，SBBR）处理含氮污水，考察了从35℃降至15℃过程的脱氮效果.结果表明，在进水氮容积负荷（NLR）为110 mg·（L·d）-1时，无纺布填料反应器的平均总氮（TN）去除率先由71.16%（35℃）提升至76.19%（30℃）后又降低至61.11%（15℃），而改性聚氨酯泡沫塑料填料的平均TN去除率先由78.71%（35℃）提升至81.75%（20℃）后又降低至75.68%（15℃）.与无纺布填料相比，改性聚氨酯填料脱氮效果更稳定，可作为优选填料.对改性聚氨酯填料生物膜进行高通量测序，结果表明主要ANAMMOX菌属为Candidatus Kuenenia，相对丰度分别为8%（35℃）、5%（25℃）和16%（15℃），可见，低温条件下，Candidatus Kuenenia仍会增殖富集.降温显著影响了生物膜群落结构. |
| 英文摘要 |
| For the ambient and low-temperature operation of the anaerobic ammonium oxidation (ANAMMOX) process, sequence batch biofilm reactors (SBBR) with non-woven fabric and modified polyurethane foam as carriers, respectively, were used to treat nitrogenous wastewater. Nitrogen removal performances of both reactors were investigated while the temperature decreased from 35℃ to 15℃. When the nitrogen loading rate (NLR) was 110 mg·(L·d)-1, the average total nitrogen (TN) removal rate of the non-woven fabric reactor increased from 71.16% (35℃) to 76.19% (30℃) and then decreased to 61.11% (15℃). The average TN removal rate of the modified polyurethane foam reactor increased from 78.71% (35℃) to 81.75% (20℃) and then decreased to 75.68% (15℃). Compared with the non-woven fabric, the modified polyurethane foam provided a more stable nitrogen removal performance and could be used as the preferred carrier. High-throughput sequencing of the modified polyurethane foam biofilm indicated that Candidatus Kuenenia was the dominant ANAMMOX genus, with relative abundances of 8% (35℃), 5% (25℃), and 16% (15℃). Candidatus Kuenenia still grew and enriched at low temperatures. The decrease in temperature significantly affected the biofilm microbial community structure. |
