| 水源水库真核微生物种群结构季相演替特征 |
| 摘要点击 2122 全文点击 682 投稿时间：2021-04-27 修订日期：2021-05-28 |
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| 中文关键词 水源水库 真核微生物 高通量测序 网络关系响应 种群季相演替 |
| 英文关键词 source water reservoir eukaryotic microorganism high-throughput sequencing network response relationship community seasonal succession |
| 作者 | 单位 | E-mail | | 张海涵 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | zhanghaihan@xauat.edu.cn | | 黄鑫 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 黄廷林 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 刘凯文 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 马曼丽 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 刘祥 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 苗雨甜 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 宗容容 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | |
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| 中文摘要 |
| 真核微生物广泛存在于水环境中且具有重要生态学功能，揭示其群落演替及其驱动因子对于研究水体生态系统的能量流动和物质循环具有重要意义.然而关于水源水库中真核微生物群落季相演替特征的研究却鲜见报道.本研究通过对李家河水库水质监测，并采用高通量测序技术和共生网络分析，探究了真核微生物种群结构的季相变化及其与水质因子的响应关系.结果表明，李家河水库水体pH处于（7.49±0.13）~（9.26±0.14），ρ（DO）最低为（7.80±0.33）mg·L-1，ρ（NH4+-N）最高为（0.31±0.01）mg·L-1，水质整体符合地表水Ⅱ类标准.真核微生物种群Shannon指数和Chao1丰富度指数夏季显著高于春秋季节.节肢动物门在春末至夏季中期为优势门，刺胞动物门和绿藻门为夏末至秋季的优势门.Telonema sp.为春末优势属（50.53%），扁藻属为夏初（30.11%）和秋初（41.34%）的优势属，Junceella sp.在夏末及秋季前中期均有较高相对丰度.杯鞭虫属（Bicosoeca sp.）、尖毛虫属（Oxytricha sp.）和壳虫属（Tintinnidium sp.）为共生网络中的关键节点，与其他真核微生物间具有显著负相关.RDA分析表明，真核微生物群落结构的季相分布受不同水质指标的调控且差异显著.本研究结果将为了解水源水库中的真核微生物群落结构动态变化及其与水环境的关系提供科学依据. |
| 英文摘要 |
| Eukaryotic micro-organisms occur extensively in the aquatic environment and play an important role in ecological functions. Therefore, revealing the succession of their communities and its driving factors is of great significance in studying the energy flow and material circulation of the water ecosystem. However, little research is available on the seasonal succession characteristics of the eukaryotic microbial community in source water reservoirs. In this study, the seasonal variation in eukaryotic community structure and its response to water quality factors were explored by monitoring the water quality of the Lijiahe Reservoir, using high-throughput sequencing technology and symbiotic network analysis. The results showed that the pH of the Lijiahe Reservoir ranged from (7.49±0.13)-(9.26±0.14), the lowest value of ρ(DO) was (7.80±0.33) mg·L-1, and the highest value of ρ(NH4+-N) was (0.31±0.01) mg·L-1. The water quality of the Lijiahe Reservoir as a whole was in accordance with the Class Ⅱ surface water standard. The Shannon index and the Chao1 richness index of the eukaryotic microbial community during the summer were significantly higher than those in spring and autumn. Arthropoda was the dominant category from late spring to mid-summer. The dominant species from late summer to autumn were members of Cnidaria and Chlorophyta. Telonema sp. was the dominant genus in late spring (50.53%) and Tetraselmis was the dominant genus in early summer (30.11%) and early autumn (41.34%). Junceella sp. had a high relative abundance in late summer and early and middle autumn. Bicosoeca sp., Oxytricha sp., and Tintinnidium sp., as key nodes, were strongly and negatively correlated with other eukaryotes. Redundancy analysis (RDA) of the eukaryotic microbial population and water quality showed that the seasonal distribution of eukaryotic microbial community structure was regulated by different water quality indexes, and there were significant differences. The results of this study will provide a scientific foundation for understanding the dynamic changes in the structure of eukaryotic microbial communities and their response to water environments in source water reservoirs. |
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