| 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 |
| 摘要点击 2338 全文点击 638 投稿时间：2020-09-26 修订日期：2020-10-06 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 粒径 比活性 微生物群落结构 基因功能预测 |
| 英文关键词 anaerobic ammonium oxidation (ANAMMOX) particle size specific activity microbial community structure gene function prediction |
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| 中文摘要 |
| 为明确基于粒径分化的厌氧氨氧化（ANAMMOX）污泥特性与菌群演替规律，研究了高氨氮生物滤池反应体系中ANAMMOX絮体污泥形成颗粒过程中的性能变化和微生物群落结构.结果表明，粒径增加显著提高了厌氧氨氧化颗粒污泥（AnGS）比活性（SAA）和耐受性，R4（>4.75 mm）的SAA最高为426.8mg·（g·d）-1，但也给传质带来不利影响.高通量测序结果显示，菌属间动态变化普遍存在.<4.75 mm时，粒径增加强化了菌群结构稳定性，絮体中含量较多的氨氧化细菌（AOB）被淘汰，脱氮比例逐渐稳定.R3（2.8~4.75 mm）具有最专一的菌群组成，功能菌Candidatus Kuenenia占比达到52.7%，而R4群落复杂度升高，功能菌占比下降，异养菌丰度增加，对颗粒结构产生负作用.此外，R3微生物的基因功能表达水平最佳，在基因复制修复和能量转化等方面明显优于小颗粒.最后通过样本间OTU矩阵分析了AnGS的进化过程.这些结果对AnGS系统的优化具有一定指导意义，将有助于ANAMMOX工艺的应用. |
| 英文摘要 |
| In order to clarify the characteristics of anaerobic ammonia oxidizing (ANAMMOX) sludge and the succession rule of bacteria based on particle size differentiation, the performance change and microbial community structure of ANAMMOX floc sludge during the formation of particles in the reaction system of a high ammonia-nitrogen biofilter were studied. The results indicated that the specific activity (SAA) and tolerance of the ANAMMOX granular sludge (AnGS) were significantly improved by increasing the particle size, and the SAA of R4(>4.75 mm) was up to 426.8 mg·(g·d)-1, but it also had adverse effects on mass transfer. The results of the high-throughput sequencing showed that dynamic changes between bacterial genera were common. When the particle size was less than 4.75 mm, the increase in particle size strengthened the stability of the bacterial flora, the ammonia oxidizing bacteria (AOB) with more flocs were eliminated, and the nitrogen removal ratio gradually stabilized. R3 (2.8-4.75 mm) exhibited the most specific flora composition, and the functional bacteria Candidatus Kuenenia accounted for 52.7%, while the R4 community complexity increased. Furthermore, the proportion of functional bacteria decreased, and the abundance of heterotrophic bacteria increased, which negatively affected the particle structure. In addition, the R3 microorganism has the best gene function expression level, which is significantly better than small particles in gene replication repair and energy conversion. Finally, the evolution of AnGS was analyzed through the OTU matrix between the samples. These results have some guiding significance for the optimization of the AnGS system and will be helpful for the application of the ANAMMOX process. |
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