| 贵州茂兰喀斯特森林不同演替下土壤真核微生物多样性 |
| 摘要点击 1515 全文点击 609 投稿时间：2020-01-02 修订日期：2020-04-02 |
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| 中文关键词 茂兰喀斯特森林 不同演替 18S rDNA 真核微生物 多样性 |
| 英文关键词 Maolan karst forest succession stage 18S rDNA eukaryotic microbes diversity |
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| 中文摘要 |
| 真核微生物是茂兰喀斯特森林土壤微生物中的重要组成部分，在生态系统物质循环和能量流动中扮演着重要角色.为了解茂兰喀斯特森林不同演替阶段下土壤真核微生物群落组成及其多样性，利用18S rDNA高通量测序技术研究了原生林（YSL）、灌木林（GML）、灌木丛（GMC）和草地（CD）这4种演替阶段下土壤真核微生物群落多样性.结果表明，在不同分类水平下，不同演替阶段土壤真核微生物群落组成相似.α多样性指数存在显著差异，Shannon指数与Simpson指数在不同演替阶段表现为：YSL > GMC > GML > CD.NMDS分析表明不同演替阶段土壤真核微生物群落结构存在差异；LEfSe分析表明，YSL中差异指示种数量高于GML、GML和CD.本研究结果为研究不同演替阶段下土壤真核微生物提供了理论基础. |
| 英文摘要 |
| As an indispensable part of the Maolan karst forest soil microorganisms, eukaryotic microbes play an important role in ecosystem material and energy flow. To investigate the composition and diversity of soil eukaryotic microbial communities under different succession stages in the Maolan karst forest, we explored the diversity and composition of soil eukaryotic microbes under four succession stages of primary forests (YSL), shrub forests (GML), bushes (GMC), and grassland (CD) via high-throughput sequencing (HTS) of the 18S rDNA. The results showed that the composition of soil eukaryotic microbial communities was similar in different succession stages under different classification levels. There was a significant difference in α diversity, Shannon, and Simpson's indices at different stages of succession as follows: YSL > GMC > GML > CD. The non-metric multidimensional scaling analysis showed that there were differences in the soil eukaryotic microbial community structure at different succession stages. The linear discriminant analysis effect size (LEfSe) analysis showed that the number of differential indicator species in YSL was higher than in GML, GML, and CD. The results of our study provide a theoretical basis for further research on soil eukaryotic microorganisms in different succession stages. |
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