| 溶藻活性物质对棕囊藻溶藻及其脂肪酸影响的模拟 |
| 摘要点击 1454 全文点击 1083 投稿时间：2015-02-05 修订日期：2015-04-24 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 芽孢杆菌 胞外活性物质 球形棕囊藻 水质参数 脂肪酸 |
| 英文关键词 Bacillus sp. algicidal substance Phaeocystis globosa water parameters fatty acid |
| 作者 | 单位 | E-mail | | 杨秋婵 | 暨南大学环境学院, 广东省普通高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | annling0513@163.com | | 赵玲 | 暨南大学环境学院, 广东省普通高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | zhaoling@jnu.edu.cn | | 尹平河 | 暨南大学生命科学技术学院, 广州 510632 | | | 谭烁 | 暨南大学生命科学技术学院, 广州 510632 | | | 舒万姣 | 暨南大学环境学院, 广东省普通高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | | | 侯少玲 | 暨南大学环境学院, 广东省普通高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | |
|
| 中文摘要 |
| 为了探讨芽孢杆菌B1分泌的胞外溶藻活性物质对球形棕囊藻的溶藻特性和藻毒素物质脂肪酸的影响,比较了模拟自然水体中叶绿素a、pH、溶解氧DO、高锰酸盐指数和营养元素N、P浓度在溶藻前后的变化,并利用GC-MS检测了球形棕囊藻脂肪酸的成分和含量. 用体积比1 :100的芽孢杆菌B1胞外活性物质处理模拟水体14 d,发现水体中叶绿素a、pH值和高锰酸盐指数随处理时间的增加而降低,DO和N、P浓度随处理时间的增加而增加. 在第14 d时,处理组水体中pH值由8.50降低到7.51,叶绿素a降低82.3% (P<0.05),DO增加29.5% (P<0.05),高锰酸盐指数降低55.2% (P<0.01). NH4+-N、NO2--N、NO3--N和PO43--P浓度分别增加了0.46、1.50、6.24 和1.30倍. 投加活性物质处理14 d后,球形棕囊藻藻毒素中的主要3种脂肪酸C18:2、C16:0和C18:1分别降低了100%、97.7%和85.4% (P<0.01),总脂肪酸含量降低83.4% (P<0.01). 结果表明,芽孢杆菌B1胞外溶藻活性物质在模拟自然水体中能有效抑制球形棕囊藻的生长,并降低藻毒素脂肪酸的含量. 研究结果为芽孢杆菌B1胞外活性物质的生态安全性应用提供理论基础. |
| 英文摘要 |
| In order to evaluate the effects of algicidal substance on Phaeocystis globosa (P. globosa) and its algal toxin -fatty acids, the changes of chlorophyll-a, pH, dissolved oxygen, permanganate index and N, P concentration were evaluated by the simulation experiment. Fatty acids composition in P. globosa was detected by GC-MS. After adding algicidal substance in simulative water with the volume ratio 1 :100, the levels of chlorophyll-a, pH and permanganate index were reduced, while the concentrations of dissolved oxygen and N, P were increased significantly within 14 days. Comparing with control group after 14 days, pH was reduced to 7.51 from 8.50, chlorophyll-a and permanganate index were reduced by 82.3% (P<0.05) and 55.2% (P<0.01), respectively. Dissolved oxygen was significantly increased by 29.5% (P<0.05). The concentrations of NH4+-N, NO2--N, NO3--N and PO43--P were respectively 0.46, 1.50, 6.24 and 1.30 times higher than that in control group. 14 days after the addition of algicidal substance, the total fatty acids of P. globosa were reduced by 83.4%. The major fatty acids C18:2, C16:0 and C18:1were reduced by 100%, 97.7% and 85.4% (P<0.01), respectively. Our results indicated that algicidal substance from Bacillus sp. B1 can effectively inhibit the growth of P. globosa and reduce the concentration of algal toxin-fatty acid in the simulation experiment. This study provides a theoretical basis for ecological safety of algicidal substance form Bacillus sp. strain B1. |
|
|
|
