| 带菌盐藻对不同形态砷的富集和转化研究 |
| 摘要点击 2736 全文点击 1202 投稿时间：2013-02-26 修订日期：2013-04-09 |
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| 中文关键词 盐藻 芽胞杆菌 砷形态 富集 转化 |
| 英文关键词 Dunaliella salina Bacillus solisalsi arsenic speciation accumulation transformation |
| 作者 | 单位 | E-mail | | 王亚 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | 2013203001@njau.edu.cn | | 张春华 | 南京农业大学生命科学实验中心, 元素与生命科学示范实验室, 南京 210095 | | | 王淑 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 申连玉 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 葛滢 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | yingge711@njau.edu.cn |
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| 中文摘要 |
| 自然环境中藻和菌多是共生的,藻菌共生体对污染环境修复具有较好的应用前景. 本研究通过16S rRNA序列分析方法从带菌盐藻中分离鉴定出1株芽胞杆菌(Bacillus solisalsi),并测定了不同浓度的亚砷酸盐[As(Ⅲ)]和砷酸盐[(As(Ⅴ)]胁迫13 d后,带菌盐藻对砷的吸收、吸附、转化情况以及培养液中的砷含量及其形态. 结果表明,无菌盐藻对砷的耐性较强,在250 μmol·L-1和500 μmol·L-1 As(Ⅲ)胁迫下,砷含量分别为3.78 g·kg-1和4.56 g·kg-1,但是培养液中的砷含量仅下降7.9%~8.3%,Bacillus solisalsi单独除砷的能力也不强(去除率为6.1%~19.9%). 盐藻及其共生菌协同除砷的能力较强,25~100 μmol·L-1 As(Ⅲ)处理下能吸收0.99~2.79 g·kg-1的砷,25~500 μmol·L-1 As(Ⅴ)处理下能吸收1.22~3.46 g·kg-1的砷. 25~100 μmol·L-1 As(Ⅲ)和As(Ⅴ)胁迫下砷去除率均在54.3%以上. 带菌盐藻可以通过As(Ⅲ)氧化、As(Ⅴ)还原、As(Ⅲ)甲基化和排出胞外等途径降低砷的毒害. |
| 英文摘要 |
| Algae and bacteria are usually symbiotic in the environment. The algae-bacteria consortia have a good prospect in the remediation of polluted environment. In this study, we isolated a bacterium from nonaxenic Dunaliella salina and identified it as Bacillus solisalsi using 16S rRNA sequence analysis. The uptake, adsorption and transformation of As by the nonaxenic D. salina and the concentration and speciation of As in the culture solution were determined after 13 days exposure to various concentrations of As(Ⅲ) and As(Ⅴ). The results showed that D. salina had a high As tolerance. When the algae was exposed to 250 μmol·L-1 and 500 μmol·L-1 arsenite, As accumulations were 3.78 g·kg-1 and 4.56 g·kg-1, respectively, but the As removal from the solution was 7.9%-8.3%. B. solisalsi did not show a strong ability to clean up As either (6.1%-19.9% removal rate). The consortia of D. salina and B. solisalsi showed a higher As removal ability. Moreover, 0.99-2.79 g·kg-1 and 1.22-3.46 g·kg-1 As were absorbed when exposed to 25-100 μmol·L-1 and 25-500 μmol·L-1 As(Ⅲ) and As(Ⅴ), respectively. More than 54.3% of As were taken away by the consortia from the solution under the exposure of 25-100 μmol·L-1 As(Ⅲ) and As(Ⅴ). Various pathways of As detoxification were identified for the nonaxenic D. salina: As(Ⅲ) oxidation, As(Ⅴ) reduction, As(Ⅲ) methylation, and efflux of As from cells. |
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