| 2,2',4,4'-四溴联苯醚的好氧微生物降解 |
| 摘要点击 1821 全文点击 1564 投稿时间：2012-08-25 修订日期：2012-10-19 |
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| 中文关键词 2,2',4,4'-四溴联苯醚 活性污泥 好氧条件 微生物降解 蛋白质组分析 |
| 英文关键词 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) activated sludge aerobic condition microbial degradation proteomic analysis |
| 作者 | 单位 | E-mail | | 张姝 | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | zhangshu_113@yahoo.cn | | Giulio Franco | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | | | 李晓豹 | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | | | 卢晓霞 | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | luxx@urban.pku.edu.cn | | 侯珍 | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | | | 杨君君 | 北京大学城市与环境学院,地表过程分析与模拟教育部重点实验室,北京 100871 | |
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| 中文摘要 |
| 从北京高碑店污水处理厂活性污泥中筛选出1株能好氧降解2,2',4,4'-四溴联苯醚(BDE-47)的细菌,并对其降解特性及有关蛋白质进行分析,目的是了解好氧条件下BDE-47的微生物降解机制. BDE-47降解菌通过平板划线法获得,其16S rDNA与不动杆菌(Acintobacter sp.)的相似度最大,为90%. 采用250 mL锥形瓶研究了所得菌对BDE-47的降解情况,在BDE-47初试浓度为146 μg·L-1的条件下,经过63 d的培养,所得菌降解了45.44%的BDE-47,降解产物主要为4-OH-联苯醚,菌量增加了7倍左右. 分别以BDE-47和酵母提取物为碳源培养所得菌2周,然后各自提取蛋白质,通过蛋白质双向电泳及质谱检测,发现了与BDE-47降解有关的一些特异蛋白质. 本研究表明,在好氧条件下,细菌可以BDE-47为碳源生长,其过程涉及多种蛋白质的作用. |
| 英文摘要 |
| By isolating a bacterial strain from the activated sludge of Gaobeidian wastewater treatment plant of Beijing and investigating its degradation property as well as the proteins involved in the degradation, this study aimed at elucidating the mechanism of microbial degradation of 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) under aerobic condition. The streak plate method was employed in the isolation of the bacteria. The 16S rDNA of the isolated bacterium had maximum similarity of 90% to Acintobacter sp.. The degradation of BDE-47 by the isolated bacterium was studied in 250 mL conical flasks. At the initial concentration of 146 μg·L-1, the bacterium degraded 45.44% BDE-47 after 63 d, the major product was 4-OH-BDE, and the bacterial mass was increased about seven times. BDE-47 and yeast extract were respectively used as the sole carbon source to enrich the bacterium for two weeks and then the proteins were extracted from the enrichments. Some specific proteins related to the degradation of BDE-47 were identified by analysis via the two dimensional electrophoresis and mass spectrometric detection. This study indicated that under aerobic condition BDE-47 can be degraded by bacteria as carbon source and multiple proteins are involved in the process. |
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