| 基于污泥资源化利用的蛋白核小球藻(Chlorella pyrenoidosa)培养研究 |
| 摘要点击 2520 全文点击 1312 投稿时间：2012-04-10 修订日期：2012-05-14 |
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| 中文关键词 蛋白核小球藻 剩余污泥 污泥抽提液 微藻培养 |
| 英文关键词 Chlorella pyrenoidosa excess sludge sludge extracts microalgae cultivation |
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| 中文摘要 |
| 利用废水或者废弃物培养微藻,不仅可使废弃物得到合理利用,还可为微藻培养提供廉价原料.以蛋白核小球藻(Chlorella pyrenoidosa)为研究对象,以污泥抽提液部分或全部替代SE(selenite enrichment)培养基,研究基于污泥资源化利用的微藻细胞培养方法.结果表明,当SE培养基与污泥抽提液比例为1∶9和2∶8时,相同条件下接种蛋白核小球藻培养14 d后,在波长为680 nm下其光密度分别为0.858和0.845,显著高于其它处理,当两者比例为0∶10和10∶0时,相应光密度分别为0.571和0.247.通过测定其色素和次生代谢产物含量时发现,当SE培养基与污泥抽提液比例为2∶8时,蛋白核小球藻的叶绿素、β-胡萝卜素和蛋白质含量最高.因此,剩余污泥抽提液可以部分作为培养蛋白核小球藻的良好基质,并且其培养效果明显优于其标准培养基.在本试验条件下,蛋白核小球藻培养的最佳条件是污泥抽提液比例为80%,该条件下蛋白核小球藻的生长状况较好,并且叶绿素与蛋白质含量最高. |
| 英文摘要 |
| Microalgae cultivation based on the waste water or other reused waste can not only make rational use of the waste, but also provide cheap materials for microalgae production. In the present study, Chlorella pyrenoidosa was used to develop a new way for microalgae cultivation based on the mix culture media with different ratio of sludge extracts and SE (selenite enrichment). Results showed that after 14 d cultivation under the same cultivating condition, the absorbency of C. pyrenoidosa at 680 nm was 0.858 and 0.845, respectively, when the ratio between culture medium of SE and sludge extracts was 1∶9 and 2∶8, and the absorbency at 680 nm was 0.247 and 0.571, respectively, when the ratio between culture medium of SE and sludge extracts was 0∶10 and 10∶0. Our results also demonstrated that highest content of chlororphyll, β-carotene and protein was achieved in C. pyrenoidosa cultivated in the mix medium between SE and sludge extracts with the ratio of 2∶8. Therefore, sludge extracts can be used as a good medium to cultivate C. pyrenoidosa, and the C. pyrenoidosa grew much better in this mix medium than in SE medium. In this study, the best condition for C. pyrenoidosa cultivation was achieved in the mix medium with 80% sludge extracts, and C. pyrenoidosa grew very well and the content of chlororphyll and protein was also high in the microalgae cell in this mixture medium. |
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