| 大气O3浓度升高对2种基因型矮菜豆丛枝菌根(AM)结构及球囊霉素蛋白产生的影响 |
| 摘要点击 1557 全文点击 679 投稿时间：2012-01-17 修订日期：2012-03-23 |
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| 中文关键词 丛枝菌根 外生菌丝 球囊霉素蛋白 泡囊 丛枝 |
| 英文关键词 arbuscular mycorrhiza external hyphae glomalin vesicule arbuscule |
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| 中文摘要 |
| 以臭氧敏感性不同的2种基因型(O3敏感型:S156; O3耐受型:R123)矮菜豆(Phaseolus vulgaris L. )为宿主植物,在模拟的大气臭氧浓度升高环境中研究臭氧胁迫对2种基因型植物的AM结构和球囊霉素蛋白产生的影响,旨在了解大气臭氧浓度升高对AM真菌生长和AM结构形成的影响. 结果表明,与自然大气臭氧水平(20 nL·L-1)相比,臭氧浓度升高(70 nL·L-1)显著降低了S156和R123植物的菌根侵染率,特别是S156植物,下降了43.6%. 臭氧浓度升高明显影响了2种基因型植物的AM结构组成,表现在根室和菌丝室外生菌丝量、单位根长丛枝数的大幅下降,以及根室和菌丝室孢子数的显著增加,特别是S156植物变化更为明显; 但2种基因型植物的单位根长泡囊数随臭氧浓度变化不显著. 臭氧浓度升高对2种基因型植物的菌根际和菌丝际总球囊霉素蛋白量影响不显著,但导致菌根际和菌丝际的易提取球囊霉素蛋白量大幅增加; 不过2种基因型植物间差异不显著. 本研究表明,大气臭氧浓度升高显著影响植物菌根侵染率、AM结构形成和易提取球囊霉素蛋白的产生,特别是对臭氧敏感型植物影响更大. |
| 英文摘要 |
| In an environment with simulated elevated atmospheric ozone, two genotypes of snap bean (Phaseolus vulgaris L. ) that differed in O3 sensitivity (O3-sensitive: S156; O3-tolerant: R123) were selected as host plants for arbuscular mycorrhizal (AM) fungi. The objective was to investigate the effect of elevated O3 on the AM structure and glomalin production in two genotypes, and to understand the effect of elevated O3on the growth of AM fungi and formation of AM structure. The results showed that in comparison with ambient O3 (20 nL·L-1), elevated O3 (70 nL·L-1) significantly decreased the mycorrhizal colonization rate in both genotypes, particularly the S156 plant (decreased by 43.6%). Elevated O3 exposed a great negative effect on the AM structure in both genotypes. For example, the arbuscule number per unit root, and the length of external hyphae in root compartment and hyphal compartment were significantly decreased, especially in S156 plant. However, the effect of elevated atmospheric O3 was not significant on the vesicule number per unit root in both genotypes. Total glomalin production in mycorrhizosphere and hyphosphere of both genotypes was only slightly affected by elevated O3, however, the production of easily extractable glomalin was significantly increased. In addition, no evident difference in glomalin concentration was observed between two genotypes at either ambient O3 or elevated O3. This study showed that the mycorrhizal colonization rate, AM structure and production of easily extractable glomalin gave great response to elevated O3, especially in the O3-sensitive plant. |
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