| 青草沙水库蓄水期间细菌群落结构变化的初步研究 |
| 摘要点击 2898 全文点击 1423 投稿时间：2011-12-19 修订日期：2012-03-19 |
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| 中文关键词 青草沙水库 PCR-DGGE 细菌群落结构 微生物计数 氮磷营养盐 |
| 英文关键词 Qingcaosha Reservoir PCR-DGGE bacterial community structure microbial culture counts nitrogen and phosphorus nutrients |
| 作者 | 单位 | E-mail | | 彭青 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | pengqingyiyi@126.com | | 谢冰 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | bxie@des.ecnu.edu.cn | | 袁琦 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | | | 黄智婷 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | | | 崔璐璐 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | | | 王文婷 | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海 200062 | |
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| 中文摘要 |
| 为调查青草沙水库蓄水及运行初期水质变化和微生物群落的关系,采用微生物培养计数和PCR-DGGE(变性梯度胶凝胶电泳)技术对水库水体中的细菌群落结构进行了研究. 结果表明,长江来水进入水库后,氮磷浓度显著降低,水质明显改善; 来水和水库中可培养微生物数量随季节变化,春夏季来水明显多于库内,秋冬季少于库内,悬浮于水中的微生物等有机体可通过沉降作用部分进入到底泥中,导致库尾底泥有机质含量增加; PCR-DGGE分析结果表明库中细菌群落结构呈现季节性变化,夏季微生物群落多样性丰度最高,秋季较低; 水库运行初期水样和底泥的微生物群落结构聚类分析的相似度可以达到62%,这与来自水中微生物的沉降有关; 来水及库中主要的优势微生物与α、β-Proteobacteria、Flavobacterium、Rheinheimera、Prochlorococcus、Synechococcus和海洋宏基因具有很高的相似性,预示着水库存在藻类规模生长和海水入侵的风险,研究结果可为水库的运行管理和深入研究提供参考. |
| 英文摘要 |
| In order to investigate the changes in water quality and the bacterial community structure in Qingcaosha Reservoir during water storage and supply period, the microorganisms in water body were studied by microbial culture counting and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DEEG) technique. Results showed that the water quality had been improved significantly and the nitrogen and phosphorus concentrations significantly reduced after the Yangtze River water flowed into the reservoir. The number of culturable microorganisms in the influent and the reservoir changed with the seasons, and there were more microorganisms in the influent than these in the reservoir during spring and summer, and fewer in autumn and winter, and the precipitation of suspended microorganisms in the water caused the increase of organic matter content in the sediment. PCR-DGGE results showed that bacterial community structure in the reservoir changed with the seasons, and the microbial community diversity was the highest in summer and the lowest in autumn. The cluster analysis showed that the similarity of microbial community structure of water and sediment samples was 62%, which might be due to the contribution of the precipitation of the suspended microorganisms. The dominant microbial species in water had high similarity with α, β-Proteobacteria, Flavobacterium, Rheinheimera, Prochlorococcus, Synechococcus and Marine metagenome, indicating that Qingcaosha Reservoir faced the risk of algae bloom and seawater intrusion. The results provide the fundamental understanding on reservoir operation and can be used as reference for future studies. |
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