| 大气O3浓度升高对2种基因型矮菜豆根际微生物的影响 |
| 摘要点击 2287 全文点击 1510 投稿时间：2011-04-13 修订日期：2011-06-07 |
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| 中文关键词 O3敏感性植物 O3耐受性植物 丛枝菌根 高浓度臭氧 微生物群落结构 微生物量 |
| 英文关键词 O3-sensitive plant O3-tolerant plant arbuscular mycorrhizae(AM) elevated O3 microbial community structure microbial biomass |
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| 中文摘要 |
| 在模拟的大气臭氧浓度升高环境中，用磷脂脂肪酸方法（PLFA）分析大气臭氧浓度升高和接种丛枝菌根（AM）真菌对臭氧敏感性不同的2种基因型矮菜豆（臭氧敏感性: S156； 臭氧耐受性: R123）根际和菌丝际微生物量及群落结构的影响，旨在明确大气臭氧浓度变化对植物根际微生物的影响，为全面评价臭氧浓度升高对土壤-植物生态系统的影响提供参考.结果表明，2种基因型植物的菌根侵染率受O3影响明显不同，S156植物的菌根侵染率在O350和80nL·L-1下明显下降，而R123植物的菌根侵染率仅在O380 nL·L-1时下降.在O3、AM、植物基因型单独及复合作用下，根际放线菌（分别受genotype、O3×AM、O3×AM×genotype影响）和菌丝际革兰氏阳性细菌的生物量（分别受O3、 AM、 AM×genotype影响）最易受影响.对于S156植物，接种AM真菌或O3浓度升高均可改变根际微生物群落结构，且两者复合作用增强了对根际微生物群落结构的影响，但O3浓度升高仅显著影响菌根化植物菌丝际的微生物群落结构.对于R123植物，O3浓度升高或接种AM真菌均能改变根际微生物的群落结构，但两者复合作用对根际微生物群落结构影响不显著；仅80 nL·L-1 O3对菌丝际微生物群落结构有显著影响.本研究表明，O3浓度升高或接种AM真菌均能不同程度地影响根际和菌丝际的微生物量和群落结构，但它们的复合影响则与宿主植物对O3的敏感性有关。 |
| 英文摘要 |
| In open top chamber (OTC), elevated atmospheric ozone was simulated to investigate effects of elevated O3 or/and arbuscular mycorrhizae (AM) inoculation on microbial biomass and microbial community structure (MCR) in rhizosphere and hyphosphere of two genotypes (O3-sensitive: S156; O3-tolerant: R123) of snap bean by phospholipid fatty acids (PLFA) method. The objective was to clarify the effect of elevated ozone on rhizosphere microorganism and provide evidence for evaluating the influence of elevated ozone on soil-plant system. The result showed that mycorrhizal colonization rates in two genotypes presented significantly different responses to elevated O3. Relative to ambient O3, mycorrhizal colonization rate of S156 plant decreased at both 50 nL·L-1and 80 nL·L-1 O3, but it only decreased at 80 nL·L-1 O3 in R123 plant. Biomass of actinomycetes in rhizosphere and Gram-positive bacteria in hyphosphere showed more sensitive to the treatments than others. Actinomycete biomass was significantly affected by plant genotypes, O3×AM and O3×AM×genotype, and Gram-positive bacterial biomass was affected O3, AM and AM×genotype. In S156 plant, AM inoculation and elevated O3 affected alone MCR in rhizosphere, and their interaction showed greater effect on MCR than single factor. But elevated O3 only altered MCR in hyphosphere of mycorrhizal plant. In R123 plant, elevated O3 and AM inoculation altered alone MCR in rhizosphere, but their interaction was slight effect on MCR. MCR in hyphosphere was only affected by 80 nL·L-1 O3. The conclusion of this study was that elevated O3 and AM inoculation can alter alone microbial biomass and community in rhizosphere and hyphosphere, but their interaction depended on sensitivity of host plant to ozone. |
