| 太湖冬季军团菌(Legionella spp.)的分布和多样性 |
| 摘要点击 2071 全文点击 1042 投稿时间：2010-09-23 修订日期：2010-11-01 |
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| 中文关键词 军团菌 太湖 DGGE 分布 基因多样性 富营养化 |
| 英文关键词 Legionella spp. Lake Taihu DGGE distribution phylogenetic diversity eutrophication |
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| 中文摘要 |
| 军团菌（Legionella spp.）广泛存在于各种水体中，对人类的健康造成了一定的安全隐患．为了解太湖在冬季是否存在军团菌，以及它的分布和多样性，本研究于2010年2月，在全太湖32个点位采样，使用巢式PCR对样品中军团菌进行检测，并用变性梯度凝胶电泳（denaturing gradient gel eletrophoresis, DGGE）及对典型条带切胶测序来分析军团菌的基因多样性．结果表明，太湖32个位点中，附着细菌和浮游细菌分别有21个点和11个点检出军团菌，检出率分别为65.63%和34.38%．附着细菌和浮游细菌DGGE图谱中，特异性条带分别有40个和36个．不同湖区的种群特征参数显示轻度富营养化湖区的军团菌种群多样性高于中度富营养化湖区．典型条带切胶回收测序共获得34个序列，以97%相似度为依据可划分为12个OTUs．切胶条带中3个序列与2种致病军团菌Legionella feeleii和 Legionella longbeachae亲缘关系很近，表明太湖中这些潜在致病菌的存在对湖区人民健康存在一定风险． |
| 英文摘要 |
| The Legionella spp. are ubiquitous in aquatic environment and could cause certain risks on human health. In order to investigate the distribution and diversity of Legionella spp. in Lake Taihu during winter time, water samples were collected from 32 sites of the whole lake in February 2010. The presence of Legionella spp. was screened by nested-PCR and their phylogenetic diversity was determined by denaturing gradient gel electrophorese(DGGE) and sequencing analysis of excised DGGE bands. The Legionella spp. was detected from 21 out of 32 sites in particle-associated bacterial samples and 11 out of 32 sites in free-living bacterial samples, which accounted for 65.63% and 34.38% of each type of bacteria, respectively. In total, 40 and 36 unique bands were identified among those particle-associated and free-living bacterial DGGE profiles, respectively. Community characteristic indices of different euthrophic areas showed that the diversity of Legionella spp. in mild eutrophic areas was higher than that in the moderate eutrophic areas. In total, thirty-four DGGE bands were excised and sequenced, which could be classified into 12 OTUs by 97% similarity. Among all the sequences, three showed a high similarity with two pathogenic Legionella species Legionella feeleii and Legionella longbeachae. This revealed potential healthy risks to the people lived around the Lake Taihu. |
