| 时间分辨荧光免疫一步法检测微囊藻毒素的研究 |
| 摘要点击 1240 全文点击 908 投稿时间：2006-08-29 修订日期：2006-09-22 |
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| 中文关键词 微囊藻毒素 时间分辨荧光免疫分析 检测 |
| 英文关键词 microcystin time-resolved fluorescence immunoassay detection |
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| 中文摘要 |
| 利用稀土元素Eu3+ 标记微囊藻毒素偶联物MCLR-BSA，在固相包被二抗的微孔板上建立了微囊藻毒素的直接竞争时间分辨荧光免疫法.条件优化后Eu3+ 标记物的稀释度为1∶50，微囊藻毒素单抗的合适浓度为100 ng/mL.该法对微囊藻毒素检测的灵敏度为0.02 ng/mL，试剂的测量范围是0.05～10 ng/mL，回收率达到94％以上. |
| 英文摘要 |
| A direct-competitive time-resolved fluorescence immunoassay(TRFIA) for microcystin detection was established, which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG. The optimal dilution of europium labeled MCLR-BSA conjugant is 1/50 and most appropriate titration of anti-microcystin-LR (MCLR) monoclonal antibodies is 100 ng/mL. The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05 ng/mL to 10 ng/mL The detection limit of the method is 0.02 ng/mL for MCLR, recoveries for microcystin (＞94%) in quantitative range is satisfactory. |
