| 基于固定化AChE的流动注射型酶传感器研究 |
| 摘要点击 1959 全文点击 1487 投稿时间：2005-10-19 修订日期：2005-12-18 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 固定化 乙酰胆碱酯酶 生物传感器 流动注射系统 有机磷农药 |
| 英文关键词 immobilization acetylcholinesterase(AChE) biosensor flow injection system organophosphorus pesticides(OPs) |
|
| 中文摘要 |
| 以固定化鲅鱼脑组织乙酰胆碱酯酶[acetylcholinesterase,AChE(EC 3.1.1.7)]为识别元件,以pH电极为换能器,构建流动注射型AChE酶传感器.该传感器在以磷酸盐缓冲液为载液的条件下具有良好的重现性(RSD=1.427%,n=10)和敏感性,可实现对有机磷化合物的在线监测;对甲基对硫磷具有线性响应的浓度范围为4.29×10-10-4.29×10-8mol·L-1,最低检测限为1.3×10- |
| 英文摘要 |
| A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius(Curier) as identification element and a pH electrode as transducer.When phosphate buffer was used as carrier liquid,a good reproducibility(RSD=1.427%,n=10) of the biosensor response was obtained after the substrate was injected repetitively.After an incubation time of 20min,the calibration graph to methyl-parathion is linear(r=0.998?6)when its concentration ranges from 4.29×10-10 mol·L-1 to 4.29×10-8 mol·L-1,and the detection limit is 1.3×10-10 mol·L-1.However,the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer.But after preoxidation of methyl-parathion with NaClO as oxidant,the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16×10-7 mol·L-1. |
