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解淀粉乳酸细菌在厨余垃圾乳酸发酵中的应用
摘要点击 1929  全文点击 1142  投稿时间:2005-04-23  修订日期:2005-05-31
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中文关键词  厨余垃圾  乳酸  解淀粉乳酸细菌  分离  发酵
英文关键词  kitchen waste  lactic acid  amylolytic lactic acid bacteria  isolation  fermentation
作者单位
王旭明 哈尔滨工业大学市政环境工程学院 哈尔滨150090 
汪群慧 哈尔滨工业大学市政环境工程学院 哈尔滨150090 
任南琪 哈尔滨工业大学市政环境工程学院 哈尔滨150090 
王孝强 哈尔滨工业大学市政环境工程学院 哈尔滨150090 
马鸿志 哈尔滨工业大学市政环境工程学院 哈尔滨150090 
中文摘要
      研究了水解淀粉乳酸细菌对厨余垃圾发酵生产乳酸的强化作用.从厌氧发酵的厨余垃圾中分离到6株水解淀粉的乳酸细菌,其中菌株FH164具有最高的淀粉降解率和乳酸产量.在pH5.5~6.0条件下,经48h发酵,菌株FH164从40.50g·L-1的可溶性淀粉产生32.67g·L-1的乳酸.根据形态、生理生化特征和16SrDNA序列分析结果,可将菌株FH164鉴定为链球菌属(Streptococcus sp.)细菌.接种菌株FH164可强化厨余垃圾的乳酸发酵,采用垃圾不灭菌的开放式发酵,菌株FH164可得到28.23g·L-1的乳酸,比自然发酵(不接种的对照)的乳酸浓度高19.2%.
英文摘要
      In order to realize resource recycling of kitchen waste,the enhancement of fermentative production of lactic acid(LA) by a strain of amylolytic lactic acid bacterium(ALAB) isolated from kitchen waste was investigated.A total of 6 strains of ALAB were isolated from anaerobically fermented kitchen waste.Among these isolates,the strain FH164 exhibited the highest starch-hydrolyzing rate and LA production.32.67g·L-1 of LA was produced from 40.50g·L-1 of soluble starch by strain FH164 at pH 5.5~6.0 in 48h of fermentation.On the basis of its morphological and physio-biochemical characteristics as well as 16S rDNA sequence,the strain FH164 was tentatively identified as Streptococcus sp..28.23g·L-1 of LA was obtained from kitchen waste by the strain FH164 employing an open fermentation mode(substrate non-autoclaved),which was 19.2% higher than that of the fermentation without inoculum(control).

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