| 氯菊酯农药间接酶联免疫吸附测定法的建立 |
| 摘要点击 1249 全文点击 1392 投稿时间：2005-01-16 修订日期：2005-03-02 |
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| 中文关键词 ELISA 拟除虫菊酯 农药 残留分析 |
| 英文关键词 ELISA pyrethroids pesticide residue analysis |
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| 中文摘要 |
| 为测定环境样品中氯菊酯农药残留,用活泼酯法将氯菊酯半抗原(Py)与卵白蛋白(OVA)偶联,制备合成抗原Py-OVA作包被原,建立间接竞争酶联免疫吸附测定法.方阵滴定确定了抗血清最佳稀释度(1∶2 500),包被抗原的最适工作浓度0.45μg/mL,并建立了标准工作曲线.工作曲线表明在10～800μg/L浓度范围内呈良好的线性关系,回收率>97%. |
| 英文摘要 |
| In order to detect the permethrin(Py) residue in environment samples, Ovalbumin(OVA)was used as protein carrier to couple with semi-antigen permethrin by active ester method.Indirect competitive ELISA(icELISA) was established.The most appropriate titration of coating antigen was 0.45μg/mL and optimal dilution was(1/2?500) correspondingly.The standard curve of icELISA was also established and the curve indicated that the lowest detection limit was 0.1μg/mL,the curve had a favorable linear relation with the concentration range of 10～800μg/mL,recoveries of permethrin(>97%) were satisfactory. |
