| 离子注入法选育高效降解蒽的鞘氨醇单胞菌突变株 |
| 摘要点击 1427 全文点击 1490 投稿时间：2004-02-24 修订日期：2004-05-17 |
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| 中文关键词 离子注入 环境微生物 原生质体 遗传稳定性 |
| 英文关键词 ion implantation environmental microorganism protoplast heritance stability |
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| 中文摘要 |
| 鞘氨醇单胞菌(Sphingomonas sp.)AN1及其原生质体经N+ 离子注入诱变处理 ,蒽的降解率分别提高了29.3%和36.2%,总变异率分别为80%～100%、60%～80% ,耐蒽浓度分别达到300mg/L、400mg/L ;突变株经15代遗传稳定性检测,有2株(AN815-3,AN315-5)的性状可稳定遗传 ,其降解率分别为73%,75%,诱变效果显著. |
| 英文摘要 |
| Sphingomonas sp. AN1 was mutagenized and breeded by N+ implantation, mutants capable of degrading anthrancene was obtained. The research review that the degrading rate increased by 29.3% and 36.2% while the mutagenized rate was 80%～100% and 60%～80%.In addition, the maximum concentration of enduring anthracene reached 300mg/L and 400mg/L. Their heritance stability were examined for fifteen transfers. Among these strains, two strains,AN815-3 and AN315-5,showed predominant character of survival ability and degrading activity. Their rate of degrading anthrancene reached 73% and 75% respectively. Effect of positive mutagenesis is evident. |
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