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南方某水厂处理工艺过程中内分泌干扰物的变化规律
摘要点击 1871  全文点击 2026  投稿时间:2003-11-03  修订日期:2004-02-11
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中文关键词  饮用水  内分泌干扰物  生物测试  样品分级  重组基因酵母
英文关键词  drinking water  EEDs  bioassay  fractionation  recombinant yeast
作者单位
饶凯锋 中国科学院生态环境研究中心环境水质学国家重点实验室 北京100085 
马梅 中国科学院生态环境研究中心环境水质学国家重点实验室 北京100085 
王子健 中国科学院生态环境研究中心环境水质学国家重点实验室 北京100085 
刘丽君 深圳市水务(集团)有限公司 深圳5180312 
中文摘要
      对南方某水厂处理工艺过程中内分泌干扰效应进行了研究.将水源水和各处理工艺的出水用固相萃取方法富集后,按不同极性洗脱,得到从非极性到极性3个组分,对总提取物和各分级富集组分分别进行重组基因酵母检测.探讨了该水厂不同处理工艺过程中内分泌干扰物的变化规律.结果表明:水源水中该类污染物浓度最高,相当于0.25 pmol/L雌二醇当量,主要存在于非极性组分(正己烷洗脱组分)中.各处理工艺段水样都存在一定程度的内分泌干扰物效应.经处理后,非极性组分内分泌干扰物效应降低,极性组分内分泌干扰物效应逐渐增加,但总地内分泌干扰物效应逐渐递减,现有工艺对雌激素类物质有较好的去除效果,去除率达到83%.研究表明,重组基因酵母检测技术结合水样的固相萃取、三步纯化分级前处理方法可以快速、有效地筛选和定量分析水样中未知内分泌干扰物及其总体效应.
英文摘要
      The variation of estrogenic effects during water treatment processes in a drinking water work in South China was investigated. Water samples were collected from the source water and the different treatment processes. Crude extracts were obtained by solid phase extraction (SPE) using HLB columns and three fractionated extracts were obtained by elution using organic reagents with different polarity. Recombinant gene yeast assay was used to evaluate the endocrine disrupters effects of all the samples. The combination of the recombinant gene yeast assay and the sample fractionation technique was effective to evaluate the estrogenic effects for water samples. All the crude samples showed positive results in the yeast assay and the highest effect was occurred in the source water sample, in which the effects were mainly attributed to the none-polar fraction and the estradiol equivalent concentration was 0.25 pmol/L. 83% estrogenic effects were reduced effectively by the treatment processes.

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