| 降解微囊藻毒素菌种的筛选和活性研究 |
| 摘要点击 2177 全文点击 2100 投稿时间：2003-12-03 修订日期：2004-02-19 |
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| 中文关键词 微囊藻毒素 微生物菌种 生物降解 滇池 |
| 英文关键词 microcystins bacterial strains biodegradation Dianchi Lake |
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| 中文摘要 |
| 研究了滇池底泥和表层水体中的微生物菌群降解微囊藻毒素(Microcystins, MCs)的能力差异,发现底泥中的微生物菌群对MCs有更强的生物降解能力.采用从滇池水华蓝藻细胞中提取提纯的微囊藻毒素作为微生物生长的唯一碳源和氮源,先后经过液体和固体培养基培养后,通过挑取单克隆菌落,分别从底泥中筛选出了能够降解MC-RR和LR的5种不同微生物菌种.其中筛选的菌种D降解MCs的能力最强,在3 d内可将初始浓度分别为60.1 mg·L-1和38.7 mg·L-1的MC-RR和LR全部降解,日均降解MC-RR和LR的速率分别高达20.0 mg·L-1和12.9 mg·L-1. |
| 英文摘要 |
| The abilities of bacterial communities, which collected from the sediment and surface water of Dianchi Lake, for the biodegradation of microcystins (MCs) were firstly investigated. It was shown that the biodegradation rates of both MC-RR and LR by bacteria in sediment were apparently higher than those by bacteria on surface water. Five strains of bacteria, which have the abilities in the biodegradation of MCs, from the sediment were isolated using the liquid and solid medium containing MC-RR and LR as the carbon and nitrogen sources, which was extracted and purified from the cells of cyanobacterial bloom. Among the bacteria isolated, bacterium D was found to have a strong ability in the biodegradation of MCs. Initial MC-RR and LR of 60.1 mg·L-1 and 38.7 mg·L-1 were completely removed in 3 days and the average biodegradation rates per day for MC-RR and LR were 20.0 mg·L-1 and 12.9 mg·L-1, respectively. |
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