| 乙草胺、甲胺磷及组合对农田黑土细菌种群生长及多样性的毒性效应 |
| 摘要点击 1768 全文点击 1643 投稿时间：2003-09-05 修订日期：2003-11-22 |
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| 中文关键词 乙草胺 甲胺磷 复合污染 细菌种群 |
| 英文关键词 acetochlor methamidophos combinated pollution bacterial population |
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| 中文摘要 |
| 应用传统及分子微生物生态学16S rDNA-PCR DGGE(Denaturing Gradient Gel Electrophoresis)等研究方法,分别对不同浓度乙草胺、甲胺磷及其乙草胺-甲胺磷二元组合胁迫下黑土中细菌活细胞数量(CFU Colony Forming Units)、种群丰富度(Richness)及其结构变化规律进行了研究.结果表明:单因子乙草胺及甲胺磷对细菌CFU产生急性毒性效应,但是长期毒性效应不显著;高浓度乙草胺-甲胺磷的二元组合对细菌CFU产生复合毒性效应,其对细菌CFU的复合抑制率明显高于相应组合中各单因子.乙草胺对可培养自生固氮菌表现出强烈刺激的作用,甲胺磷表现为严重抑制作用;而所有乙草胺-甲胺磷二元组合却对自生固氮菌CFU表现为复合毒性效应,其复合毒性明显高于相应组合中各单因子对自生固氮菌的毒性效应.应用16S rDNA-PCR DGGE技术研究结果显示:乙草胺、甲胺磷及乙草胺-甲胺磷组合不同程度地降低了黑土中细菌种群在分子水平上的丰富度,并使其种群组成和结构均发生了显著变化. |
| 英文摘要 |
| Bacterial amount and population richness in agricultural black soil under the stress of acetochlor, methamidophos and their combination were analyzed by CFU and 16S rDNA-PCR DGGE.Results showed that both of acetochlor and methamidophos had acute toxic effects on the growth of bacteria in agricultural black soil;Growth of free-living nitrogen fixing bacteria was inhibited badly by methamidophos ,but stimulated greatly by acetochlor. The joint inhibitory effects of all combinations at different concentration between methamidophos and acetochlor on free-living nitrogen fixing bacteria were severer than each of their singal factors,which indicate the siqnificant joint toxic effects between acetochlor and methamidophos on free-living nitrogen fixing bacteria in tested soil. Bacterial population diversity were decreased and the structure were changed respectively at different levels according to their concentration and combinations.Some bacterial species were diminished , but some others were accumulated under the stress of two agrochemicals and their combinations. |
