| 卤代烃毒性的化学评价法:鸟嘌呤卤代烃加合物FTIR、UV光谱表征 |
| 摘要点击 847 全文点击 1664 投稿时间：2000-02-21 |
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| 中文关键词 鸟嘌呤 加合物 异构体 FTIR |
| 英文关键词 guanine adduct isomer FTIR |
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| 中文摘要 |
| 研究鸟嘌呤与一系列卤代烃反应的加合产物 ,利用HPLC二极管阵列检测器得到的特征紫外光谱 (UV)对加合产物样品进行了鉴定 ,然后加合产物经薄层 (TLC)分离 ,再进一步做红外 (FTIR)鉴定 ,从而得到鸟嘌呤 卤代烃氧位和氮位加合物的红外光谱表征 .研究表明 ,鸟嘌呤卤代烃的氮位加合物异构体的紫外光谱吸收峰的波长在 240～250nm之间 ,氧位加合物UV波长在 260～270nm附近 .鸟嘌呤与本文涉及的各卤代烃的加合物的红外光谱之间差别不大 :其氮位加合物在 1700cm-1处 ,均有CO的特征吸收峰 ;其氧位加合物的红外光谱亦相近 . |
| 英文摘要 |
| Determination and identification of guanine halohydrocarbon adduct isomers were carried out by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR).The experimental results showed that the UV spectral overlay of O6 guanine adducts retrieved after HPLC at 260～270nm.The UV spectra of N side adducts are similarly between 240～250nm.After thin layer chromatographic separation (silica gel-GF254),the all IR spectra of the N side adducts of guanine with different halohydrocarbons showed a absorption near 1700cm-1 assigned the characteristic peak of CO.The IR spectra among the adducts of O6 guanine with different halohydrocarbons are quite similar. |
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