| 硫酸盐还原菌对多种染料厌氧脱色的特性 |
| 摘要点击 750 全文点击 1679 投稿时间：1997-12-26 |
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| 中文关键词 硫酸盐还原菌 染料 厌氧脱色 硫化氢 |
| 英文关键词 sulfate-reducing bacteria dyes anaerobic decolorization H2S |
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| 中文摘要 |
| 研究专性厌氧菌硫酸盐还原菌混合培养物对偶氮染料、三苯甲烷染料、蒽醌染料的脱色作用。在菌的生长过程中,随着硫化氢的形成,染料的脱色率不断增长。分别测定了完整细胞和上清液对5种染料的脱色能力。当上清液中含有硫化氢时,在1h内不同染料(50mg/L)的脱色率可达25.58%-98.8%;而完整细胞的脱色率则为6.99%-80.60%.由此表明,在厌氧硫酸盐还原条件下,硫酸盐还原菌及其产物硫化氢对染料的脱色均有重要作用。完整细胞对10种结构不同的染料(50mg/L)的半脱色时间为0.5-32.6h,甚至更长,脱色的最适温度和pH分别为30℃和8.0,严格厌氧;还原型烟酰胺腺嘌呤二核苷酸对脱色具有促进作用,粗酶液对酸性红B2GL脱色比活(染料/蛋白)为0.87μg/(mg·min),米氏常数Km值为14mmol/L. |
| 英文摘要 |
| The decolorization of azo dyes,tribenzic methane dyes and anthracene dyes by anaerobic bacteria,sulfate-reducing bacteria(SRB) were studied in this paper.In the anaerobic growth of SRB,the decolorization rates of dyes increased with the forming of H2S.The decolorizing abilities of intact cells and supernatant for 5 dyes were also determined,the results showed that the decolorization rates of these dyes(50mg/L) by supernatant containing H2S were 25.58%—98.8% within reaction for 1h,and those by intact cells were 6.99%—80.60%.It was found that both of cells and H2S producing from the bacteria acted important roles in the decolorization of dyes.The half decolorization times for 10 different dyes by intact cells lay from 0.5 to 32.6h or more.The optimum temperature and pH were 30℃ and 8.0,respectively.Reduced nicotinamide adenine dinucleotide(NADH) could improve the decolorizing activities.The specific activity and Michaclis Menten Constant(Km) value of crude enzyme for Acid Red B2GL were 0.87μg/(mg·min) and 14mmol/L,respectively. |
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